Exploring pericyclic reactions for bioorthogonal imaging of biological processes with molecular precision

Projektinformationen

PERBIOIMAGE

ID Finanzhilfevereinbarung: 630731

Projekt abgeschlossen

Startdatum 1 April 2014

Enddatum 31 März 2018

Finanziert unter

• Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Gesamtkosten € 100 000,00

EU-Beitrag € 100 000,00

Koordiniert durch

INSTITUTO DE MEDICINA MOLECULAR JOAO LOBO ANTUNES
Portugal

Ziel

Bioorthogonal reactions have become essential tools for Chemical Biology and Drug Discovery. Despite recent advances in the development of bioorthogonal reactions, there still exists a need for reactions with improved kinetics and selectivities. Alkenes have been exploited in the context of site-specific protein modification and labelling, for example in cross-metathesis, photochemical 1,3-dipolar cycloaddition and tetrazine Diels-Alder cycloaddition strategies. Pericyclic reactions are a class of reactions where alkenes are suitable partners to achieve selectivity. These reactions are particularly attractive for applications in biological systems because their often-accelerated rate in polar media and their concerted mechanisms reduce undesired reactivity with biological nucleophiles and electrophiles. This project aims to 1) explore the reactivity of alkenes in benign aqueous conditions for site-specific ligand/protein labelling using a photoinduced [2+2] cycloaddition strategy, 2) site-specific incorporation of bioorthogonal group norbornadiene via genetic code expansion for directed site-specific cellular protein labeling, 3) the design and synthesis of ligands of ligands of oncogenic IL7R in T-cell acute lymphoblastic leukaemia (T-ALL) and 4) the use of a bioorthogonal approach for live cell imaging of oncogenic IL7R in T-ALL.

Wissenschaftliches Gebiet

• medical and health sciences › basic medicine › pharmacology and pharmacy › drug discovery
• natural sciences › biological sciences › biochemistry › biomolecules › proteins
• medical and health sciences › clinical medicine › oncology › leukemia

Programm/Programme

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Thema/Themen

• FP7-PEOPLE-2013-CIG - Marie-Curie Action: "Career Integration Grants"

Aufforderung zur Vorschlagseinreichung

FP7-PEOPLE-2013-CIG
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

MC-CIG - Support for training and career development of researcher (CIG)

Koordinator

INSTITUTO DE MEDICINA MOLECULAR JOAO LOBO ANTUNES

Kontakt Verwaltung

Fausto Carvalho (Mr.)

• EU-Beitrag: € 100 000,00
• Adresse: AVENIDA PROF EGAS MONIZ ; 1649 028 Lisboa ; Portugal
• Region: Continente › Área Metropolitana de Lisboa › Área Metropolitana de Lisboa
• Aktivitätstyp: Research Organisations
• Gesamtkosten: Keine Daten