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Esrrb Function in Mitotic Bookmarking

Objective

The preservation of cell identity depends on the maintenance of gene expression programs through mitosis. Generally, this is mediated by epigenetic systems of repression involving Polycomb proteins and H3K9 and DNA methyltransferases. Pluripotent Embryonic Stem (ES) cells constitute an exception as abrogation of these systems does not lead to detrimental consequences, with defects appearing only during differentiation. This is particularly surprising since ES cells maintain their transcriptome and developmental potential over long periods of frequent divisions. Understanding how ES cells identity is mitotically maintained is the focus of this application.
An unconventional view will be adopted in which the mitotic stability of pluripotency depends on the memory of gene activation (rather than repression). These so-called “bookmarking mechanisms” rely on transcription factors whose binding at specific genomic locations is mitotically stable, preserving and instructing gene activity from mother to daughter cells. The major hypothesis driving this proposal is therefore that memory of active transcription in ES cells is maintained by retention of one or several pluripotency transcription factors on mitotic chromatin. We have identified one such “bookmarking” factor and we propose to study its potential function on the intergenerational maintenance of ES cells identity.

Call for proposal

FP7-PEOPLE-2013-IEF
See other projects for this call

Coordinator

INSTITUT PASTEUR
EU contribution
€ 194 046,60
Address
Rue du docteur roux 25-28
75724 Paris
France

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Region
Ile-de-France Ile-de-France Paris
Activity type
Research Organisations
Administrative Contact
Marie-Laure Rosso (Dr.)
Links
Total cost
No data