Objective
Posttranscriptional gene regulation is an essential process exerted by a multitude of RNA-binding proteins (RBPs) and allows for rapid adaptation of the cellular proteome to environmental perturbations. Previously, only in a limited number of instructive cases, the RNA networks targeted by individual RBPs had been elucidated. The recent proteome-wide unbiased identification of RBPs yielded a comprehensive catalogue of RBPs acting in human cells and has assigned an RNA-binding activity to over 300 orphan proteins, however without revealing their biological function. FASTKD (FAS-activated serine/threonine kinase domain-containing) proteins constitute an entire family of newly assigned RBPs with poorly defined functions and lack classical RNA binding domains. A loss of function mutation within FASTKD2 has been associated with a lethal form of infantile encephalomyopathy. FASTKD2 localizes to mitochondria and appears to regulate mitochondrial homeostasis by entirely undefined mechanisms. To address to which extent and how RNA binding contributes to the evidently vital functions of FASTKD2 I will extensively characterize the FASTKD2-RNA interface. I will determine the regions of FASTKD2 that interact with RNA making use of a novel and unprecedented technique termed RBDmap. The results of this analysis will guide systematic tests of RNA binding mutants of FASTKD2 in RNA interference based complementation assays. To obtain a comprehensive picture of the factors mediating the effects associated with a loss of function of FASTKD2 I will analyze the network of proteins and RNA motifs associating with FASTKD2 at the systems level. The models derived from the obtained data will be validated in cell-based assays. The required interdisciplinary approach is unique and will yield valuable insights into the molecular actions and interconnections of an entirely new type of RNA binding proteins in mitochondrial homeostasis in health and disease.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences genetics mutation
- natural sciences biological sciences genetics RNA
- medical and health sciences basic medicine physiology homeostasis
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2013-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
69117 Heidelberg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.