High throughput analysis of the in vivo metabolite/protein interactome in plants

Projektinformationen

TAPMIN

ID Finanzhilfevereinbarung: 627033

Abgeschlossenes Projekt

Startdatum 1 Juni 2014

Enddatum 31 Mai 2016

Finanziert unter

• Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Gesamtkosten € 235 000,00

EU-Beitrag € 235 000,00

Koordiniert durch

VIB VZW
Belgium

Ziel

In the last decade, new technologies have emerged to study protein-protein and protein-nucleic acid interactions under near-physiological conditions at large scale. In contrast, development of similar performing technologies for screening and discovery of in vivo metabolite/protein interactions has received little attention. Nonetheless, metabolites comprise a large and important fraction of the cellular molecules. Many can bind proteins as substrates, products, cofactors, and ligands, and thereby constitute metabolic networks or regulate a myriad of environmental and developmental responses, often at very low concentrations. Here, we will establish a performing method for the discovery of novel in vivo protein-metabolite interactions in the model plant Arabidopsis thaliana by adapting an available high throughput protein affinity purification/mass spectrometry platform used for detecting protein-protein interactions. Receptor proteins involved in jasmonate signalling will be used as the model systems to discover new metabolites that interact in vivo with proteins. Ultimately, the role of the identified metabolites and the relevance of their molecular interactions for the jasmonate signalling cascades that steer growth and metabolism will be investigated.

Wissenschaftliches Gebiet

• natural sciences › biological sciences › biochemistry › biomolecules › proteins › proteomics
• natural sciences › chemical sciences › analytical chemistry › mass spectrometry

Programm/Programme

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Thema/Themen

• FP7-PEOPLE-2013-IEF - Marie-Curie Action: "Intra-European fellowships for career development"

Aufforderung zur Vorschlagseinreichung

FP7-PEOPLE-2013-IEF
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

MC-IEF - Intra-European Fellowships (IEF)

Koordinator

VIB VZW

Kontakt Verwaltung

Rik Audenaert (Mr.)

• EU-Beitrag: € 235 000,00
• Adresse: SUZANNE TASSIERSTRAAT 1 ; 9052 ZWIJNAARDE - GENT ; Belgien
• Region: Vlaams Gewest › Prov. Oost-Vlaanderen › Arr. Gent
• Aktivitätstyp: Research Organisations
• Gesamtkosten: Keine Daten