Objective Single Nano-Particles undergoing Brownian motion in solution colliding at an inert ultramicroelectrode, can serve as nano-electrodes during the time of contact. This provides an entirely new approach to nanoelectrochemistry. Hitherto, this new technique has been used for understanding simple redox reactions. We propose to experimentally study the electron transfer kinetics of single redox active proteins under steady state diffusion conditions, in this elegant configuration. In this way realistic mimicking of electron transfer based natural events will be achieved. In addition, the fundamentals of biological electron transfer will be revisited and re-evaluated. In parallel to this, we plan to use the natural redox activity of selected amino acids within a protein to establish a simple type of biosensor, with approximately attomolar detection sensitivity for label free proteins. Recently, the ability to observe electrocatalytic activity of single NP’s (due to collision with an electrode) was established. In the same way, we plan to use proteins as nanoparticles for biosensing applications. By immobilizing specific aptamers on an inert substrate, the absorption of label free proteins on top of the aptamers will be tracked. Chronoamperometric profiles measured on ultramicroelectrode under potential control should allow detection of attomolar concentrations. Fields of science engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsbiosensorsnatural scienceschemical sciencesorganic chemistryorganic reactionsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsengineering and technologynanotechnologynano-materialsnatural scienceschemical sciencesorganic chemistryamines Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) FP7-PEOPLE-2013-IEF - Marie-Curie Action: "Intra-European fellowships for career development" Call for proposal FP7-PEOPLE-2013-IEF See other projects for this call Funding Scheme MC-IEF - Intra-European Fellowships (IEF) Coordinator THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD EU contribution € 231 283,20 Address WELLINGTON SQUARE UNIVERSITY OFFICES OX1 2JD Oxford United Kingdom See on map Region South East (England) Berkshire, Buckinghamshire and Oxfordshire Oxfordshire Activity type Higher or Secondary Education Establishments Administrative Contact Gill Wells (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
