Objective
Centrosomes are the main microtubule-organizing centers in animal cells and their structure and function are extremely well conserved across evolution. Centrosome localization is a dynamic, cell cycle, and cell type regulated process that plays many critical roles in a variety of cell functions and animal development. I have recently published, as part of my work in the lab of Pierre Gönczy, a siRNA genome wide screen that has successfully identified new regulators of centriole duplication. In this work we have used a HeLa cell line stably expressing the centriolar protein Centrin-1 tagged to GFP. We have developed an algorithm to automatically identify GFP positive foci and use it to measure among other features centriole number. Remarkably, there is information that was extracted from the collection of images that remain to be analyzed and that can reveal new features about centrosome biology. One exciting example is the analysis of centriole position within the cell. I aim to use the data to identify new candidates required for proper centrosome localization in a genome-wide scale. The more straightforward way to achieve this goal will be to identify those conditions where centrosome position is not in close proximity to the nucleus. As a proof of principle I have searched in our database for genes previously reported in literature to influence centrosome-nucleus connection. Preliminary visual inspection showed examples where the percentage of cells displaying an increased nucleus-centriole distance was significantly higher than in control conditions (i.e. BICD2, CENP-F, DCTN1, Nesprin-3 and SUN-1). This preliminary data encourage me to embark on a more systematic and quantitative search of candidates in a genome-wide scale. In order to perform this project I will relocate to a more experience lab in the field of organelle positioning and centrosome-organelle interaction as it is the case of Dr. Rosa M. Rios laboratory located in a leading research institution.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences databases
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2013-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
28006 MADRID
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.