Obiettivo Understanding how and where our memory is stored is a long sought-after task. It is accepted that proteins at synapses play a role in capturing and maintaining memory. It is now clear that synapses possess the capacity for local protein synthesis, and that dendritic protein synthesis is required for many forms of long-term synaptic potentiation (LTP) and plasticity.It remains unclear over what spatial scale local translation can be regulated and stimulated. Moreover, the location of specific translation sites and the mechanisms by which local dendritic translation is activated are largely unknown.This study aims to determine the effect of single spine activity on local protein synthesis, and to decipher how varying levels of activation of the synapse activity in adjacent spines alters the dynamics and trafficking of newly synthesized proteins and to what extent (spatially and temporally).I will examine the correlation between local translation and the following neurobiological processes: synaptic plasticity, LTP, and the hypothesis of synaptic tagging and capturing. Their activation will be induced using different levels and patterns with high resolution glutamate uncaging, in adjacent or remote spines including on different dendritic branches.I propose to decipher the location of protein synthesis using fluorescent non-canonical amino acid tagging, which allows visualizing the protein synthesis in real time and in its natural site. I will address the translational regulation of exemplar proteins, of which their mRNAs were found to localize in dendrites, that represent neurotransmitter receptors (e.g. GluA1 or 2), scaffolding molecules (e.g. Shank, PSD-95), signaling molecules (e.g. CaMKIIa) and cytoskeletal elements (e.g. b-actin).Understanding the spatial and temporal synthesis of proteins following different levels and patterns of spinal activity will shed light on the mechanisms by which important cell biology processes including LTP and plasticity occur. Campo scientifico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencescell biologynatural scienceschemical sciencesorganic chemistryamines Programma(i) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) FP7-PEOPLE-2013-IEF - Marie-Curie Action: "Intra-European fellowships for career development" Invito a presentare proposte FP7-PEOPLE-2013-IEF Vedi altri progetti per questo bando Meccanismo di finanziamento MC-IEF - Intra-European Fellowships (IEF) Coordinatore MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV Contributo UE € 161 968,80 Indirizzo HOFGARTENSTRASSE 8 80539 Munchen Germania Mostra sulla mappa Regione Bayern Oberbayern München, Kreisfreie Stadt Tipo di attività Research Organisations Contatto amministrativo Mitra Moghadassian (Mrs.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato
