Obiettivo 50 years after the discovery of the DNA double helix, the variety of structures that nucleic acids can adopt continues to surprise the scientific community. Specific structures and conformational changes are linked to important functions in cell regulation. Understanding the principles that govern how small molecules such as natural metabolites or synthetic drugs modulate the nucleic acid structures is of prime importance for molecular biology and pharmacology. The field however suffers from the lack of suitable experimental tools to monitor all assemblies and structures formed when a small molecule encounters its targets.The goal of my project is to develop unique mass spectrometry-based approaches to detect, quantify and characterize all these assemblies and structures. Our team’s strength will be to integrate a multidisciplinary approach, from physical and analytical chemistry to molecular biology. We will address the fundamentals of nucleic acid ionization and transfer in the gas phase, develop a unique instrumental setup combining mass spectrometry, ion mobility and circular dichroism ion spectroscopy, and apply these new approaches to biologically important nucleic acids, in order to reveal the mechanisms of ligand-induced conformational changes in important regulatory structures such as G-quadruplex or riboswitches.This research will also have broader impact, as the approaches and concepts developed here for nucleic acids will contribute fundamental advances in mass spectrometry, and will be transferrable to other supramolecular or biological complexes. Campo scientifico natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencesgeneticsDNAnatural scienceschemical sciencesanalytical chemistrymass spectrometrynatural sciencesphysical sciencesopticsspectroscopynatural sciencesbiological sciencesmolecular biology Programma(i) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) ERC-CG-2013-PE4 - ERC Consolidator Grant - Physical and Analytical Chemical Sciences Invito a presentare proposte ERC-2013-CoG Vedi altri progetti per questo bando Meccanismo di finanziamento ERC-CG - ERC Consolidator Grants Istituzione ospitante INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE Contributo UE € 2 021 755,20 Indirizzo RUE DE TOLBIAC 101 75654 Paris Francia Mostra sulla mappa Regione Ile-de-France Ile-de-France Paris Tipo di attività Research Organisations Ricercatore principale Valérie Gabelica (Dr.) Contatto amministrativo Jérémy Bonnassies (Mr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato Beneficiari (1) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE Francia Contributo UE € 2 021 755,20 Indirizzo RUE DE TOLBIAC 101 75654 Paris Mostra sulla mappa Regione Ile-de-France Ile-de-France Paris Tipo di attività Research Organisations Ricercatore principale Valérie Gabelica (Dr.) Contatto amministrativo Jérémy Bonnassies (Mr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato
