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Content archived on 2024-06-18

Simultaneous Detection of Multiple DNA and Protein Targets on Paramagnetic Beads Packed in Microfluidic Channels using Quantum Dots as Tracers

Objective

The detection of DNA hybridization and protein recoginittion event (immunoassay) is very important for the diagnosis and treatment of genetic diseases, for the detection infectious agents and for reliable forensic analysis. Recent activity has focused on the development of hybridization assays that permit simultaneous determination of multiple DNA or protein targets, using optical or electrochemical coding technology, based on unique encoding properties of semiconductor crystal nanoparticle tags (quantum dots). Described multi-target bio assays were performed in batch mode, involving significant amount of steps, connected with the possibility of human error, time and reagents consuming. Lab-on-a-chip technology offers tremendous potential for obtaining desired analytical information in a simpler, faster and cheaper way compared to traditional batch/laboratory-based technology. Particularly attractive for multiple DNA and protein recognition applications (i.e. point-of-care) is the high-throughput, automation, versatility, portability, reagent/sample economy and high-performance of such micromachined devices. Overall objective of the proposed research is to create and characterize a portable microanalyzer, based on a novel advanced Lab-on-a-Chip technology with magnetic separation and end-column quantum dots tracers voltammetric detection of multiple DNA and protein targets for point-of-care , automated, high-throughput, sensitive, selective and simultaneous assays. The new micro-total analytical system will rely on coupling of microfluidic transport of samples, effective flow-through magnetic separation complementary/non-complementary DNA and protein targets and a novel chip-based voltammetric stripping detection of quantum dot tags. To successfully complete such advanced micro-total analytical system, several fundamental and practical issues will be addressed.

Fields of science (EuroSciVoc)

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Topic(s)

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

ERC-2009-StG
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

ERC-SG - ERC Starting Grant

Host institution

ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE
EU contribution
€ 1 400 000,00
Address
BATIMENT CE 3316 STATION 1
1015 LAUSANNE
Switzerland

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Region
Schweiz/Suisse/Svizzera Région lémanique Vaud
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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Beneficiaries (1)

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