BeyondSeqProject ID: 634890
Genomic diagnostics beyond the sequence
Total cost:EUR 6 034 026,09
EU contribution:EUR 6 034 026,09
Topic(s):PHC-10-2014 - Development of new diagnostic tools and technologies: in vitro devices, assays and platforms
Call for proposal:H2020-PHC-2014-two-stageSee other projects for this call
Funding scheme:RIA - Research and Innovation action
Cytogenetic diagnostic approaches provide information on the single-chromosome level but suffer from low resolution and throughput. In contrast, next generation sequencing (NGS) based diagnostics provides single base resolution and high throughput but suffer from short reads that prevent analysis of large genomic aberrations as well as being prone to PCR-amplification bias and erasure of epigenetic information. This proposal aims to bridge the gap between these domains by analyzing long individual DNA molecules without PCR-amplification via utilization of emerging optical DNA mapping technologies. We specifically address three types of challenges to current genomic based diagnostics:
1. Loss of relevant information such as DNA damage lesions, rare mutations or epigenetic markers following PCR amplification.
2. Limitations in resolving long-range variations in genomic layout and correlating them with single point mutations, preventing large scale screens.
3. Limitations imposed by the sample such as low sample amounts (micro biopsy, circulating tumor DNA) or inhomogeneous/highly variable samples (bacterial cultures).
We will develop a robust toolbox for integrated genetic and epigenetic profiling of single DNA molecules that will include automated sample preparation of native unamplified DNA as well as the hardware and software platforms and analysis tools for readout, extraction and quantification of medically relevant genomic information. This technology will be used to develop a set of specific, proof of principle diagnostic assays based on optical barcoding of individual DNA molecules. These assays will address:
-Bacterial infections and antibiotic resistance
-Diagnosis/prognosis tools for hematological malignancies
-Spinal Muscular Atrophy
-Early diagnosis of colorectal and lung cancer.
Ultimately our project will provide reagents, prototype DNA barcoding devices and data analysis software ready for large scale validation and early stage commercialization.
EU contribution: EUR 1 147 250
69978 TEL AVIV
EU contribution: EUR 840 361,25
EU contribution: EUR 799 863,75
EU contribution: EUR 844 475
Technion City-Senate Building
EU contribution: EUR 778 312,49
Oude Markt 13
EU contribution: EUR 674 497,50
Rue du Faubourg Saint Jacques 29
EU contribution: EUR 703 578,75
B15 2TT BIRMINGHAM
EU contribution: EUR 245 687,35
43-45 DORSET STREET 2ND FLOOR
W1U 7NA LONDON