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The Crosstalk Between Red And White Blood Cells: The Case Of Fish

Project description

Understanding the role of red blood cells in immune response in fish

Recent studies demonstrated processes relevant to immunity in the red blood cells (RBCs) of diverse organisms, which included pathogen recognition, binding and clearance, and cytokines production. Furthermore, it was found that nucleated fish and avian RBCs develop specific responses to pathogen molecular patterns and produce soluble factors that modulate leukocyte activity. The ERC-funded BLOODCELLSCROSSTALK aims to uncover the immune mechanisms of fish protection against viral infections, with a focus on the nucleated fish erythrocytes as the potential key mediators of the antiviral responses. The objective is to evaluate the crosstalk between red and white blood cells during fish viral infections and prophylaxis, using a working model of the rainbow trout and the viral haemorrhagic septicaemia virus.

Objective

Fish are the phylogenetically oldest vertebrate group with an immune system with clear similarities to the immune system of mammals. However, it is an actual matter of fact that the current knowledge of the fish immune system seems to lack the key piece to complete the puzzle.
In 1953 Nelson described a new role of human red blood cells (RBCs) which would go beyond the simple transport of O2 to the tissues. This new role, involved in the defence against microbes, described the antibody and complement-dependent binding of microbial immune complexes to RBCs. Regardless of the importance of this finding in the field of microbial infection, this phenomenon has been poorly evaluated. Just recently, a set of biological processes relevant to immunity have been described in the RBCs of a diverse group of organisms, which include: pathogen recognition, pathogen binding and clearance and cytokines production. Furthermore, it has been demonstrated that nucleated erythrocytes from fish and avian species develop specific responses to different pathogen associated molecular patterns and produce soluble factors that modulate leukocyte activity.
In the light of these pieces of evidences, and in an attempt to improve the knowledge of the immune mechanism(s) responsible for fish protection against viral infections, we raised the question: could nucleated fish erythrocytes be the key mediators of the antiviral responses? To answer this question we decided to focus our project on the evaluation of the crosstalk between red and white blood cells in the scenario of fish viral infections and prophylaxis. For that a working model composed of the rainbow trout and the viral haemorrhagic septicaemia virus (VHSV) was chosen, being the objectives of the project to evaluate: i) the implication trout RBCs (tRBCs) in the clearance of VHSV, and ii) the involvement of tRBCs in the blood transportation of the glycoprotein G of VHSV (GVHSV), the antigen encoded by the DNA vaccine.

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Topic(s)

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ERC-STG - Starting Grant

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Call for proposal

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(opens in new window) ERC-2014-STG

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Host institution

UNIVERSIDAD MIGUEL HERNANDEZ DE ELCHE
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 823 250,00
Address
AVENIDA DE LA UNIVERSIDAD S-N
03202 Elche
Spain

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Region
Este Comunitat Valenciana Alicante/Alacant
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 823 250,00

Beneficiaries (1)

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