Objectif This Proof of Concept proposal is designed to develop and test a novel way of monitoring the effects of drug binding to membrane protein targets. The idea stems from the basic research proposed in my ERC grant IMPRESS to study and rationalise the release of membrane proteins from detergent micelles in the gas phase of the mass spectrometer. During this research we discovered a new family of detergents, which we could formulate, to retain folded protein structure in the gas phase. Having achieved this goal, we then actively unfolded these assemblies in the mass spectrometer and by monitoring their unfolding trajectories we realised that we could quantify the effects of small molecule binding on protein stability. Not only can we now rank ligands according to their ability to confer stability but we can also count the number of molecules bound to a particular membrane protein target. By distinguishing bound molecules, either lipids or drugs, we can then carry out competitive binding experiments by displacing small molecules with higher affinity ligands. Champ scientifique natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesbiochemistrybiomoleculeslipids Programme(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Thème(s) ERC-PoC-2014 - ERC Proof of Concept Grant Appel à propositions ERC-2014-PoC Voir d’autres projets de cet appel Régime de financement ERC-POC - Proof of Concept Grant Coordinateur THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Contribution nette de l'UE € 145 501,00 Adresse Wellington square university offices OX1 2JD Oxford Royaume-Uni Voir sur la carte Région South East (England) Berkshire, Buckinghamshire and Oxfordshire Oxfordshire Type d’activité Higher or Secondary Education Establishments Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 145 501,25 Bénéficiaires (1) Trier par ordre alphabétique Trier par contribution nette de l'UE Tout développer Tout réduire THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Royaume-Uni Contribution nette de l'UE € 145 501,00 Adresse Wellington square university offices OX1 2JD Oxford Voir sur la carte Région South East (England) Berkshire, Buckinghamshire and Oxfordshire Oxfordshire Type d’activité Higher or Secondary Education Establishments Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 145 501,25