MATRIXASSAYProject ID: 644175
Novel Cell Migration Assay Based on Microtissue Technology and Tissue-Specific Matrices
Total cost:EUR 1 053 000
EU contribution:EUR 931 500
Call for proposal:H2020-MSCA-RISE-2014See other projects for this call
Funding scheme:MSCA-RISE - Marie Skłodowska-Curie Research and Innovation Staff Exchange (RISE)
Cell migration assays are commonly used to study wound healing, cancer cell invasion, and tissue development. Problems associated with the gap closure assays typically employed are that:
(i) the stopper or scratch used to make the migration zone damages the extracellular matrix (ECM),
(ii) the migration zone size is limited by the size of the stopper, and
(iii) the scratched migration zone shapes and sizes are irreproducible. Cell migration is strongly coupled with the structure and mechanical properties of the ECM, and damage to the ECM alters the cell migration path.
The main objective of this project is to develop a prototype novel cell migration assay, which will significantly improve the predictive power of cell-based assays while avoiding problems associated with existing assays, based on seeding cells precisely on pristine extracellular matrix tissue mimics with native-like cell-functionality and reproducible migration zones.
In accomplishing this, we will also address the following questions:
• What are the structure-property relationships between collagen I matrices with controlled thicknesses and fibril diameter and alignment, and their mechanical and electromechanical properties?
• What are the critical parameters for achieving functional bonding between the substrate and the highly anisotropic viscoelastic collagen I matrices and controlling the overall mechanical properties?
• Does the distribution of collagen fibril polar ordering, i.e., piezoelectric domains, influence cell migration?
• What parameters control crimp formation in tendon-like collagen I matrices?
• What parameters control and explain the unusual viscoelastic properties (e.g., they not depend on the speed of deformation, at least within the interval 0.01 - 1 mm/sec) of tendon-like collagen matrices?
• Which cell types, including cancer cells, co-align with collagen fibril alignment or crimp direction?
EU contribution: EUR 216 000
EU contribution: EUR 310 500
King Street, Old College
EU contribution: EUR 324 000
LE11 3TU LOUGHBOROUGH
EU contribution: EUR 54 000
UPPER NEWCASTLE ROAD, BUSINESS INNOVATION CENTRE, NATIONAL UNIVERSITY OF IRELAND GALWAY
EU contribution: EUR 27 000
msc house/lyon way
GU16 7ER FRIMLEY, CAMBERLEY SURREY