Objective Meiotic recombination is an essential process for sexually reproducing organisms. The appropriate genomic distribution of recombination events is necessary for the accurate transmission of genetic information from one generation to the next. Extensive research has demonstrated that a major determinant of recombination sites in mice and humans is the DNA-binding protein PRDM9. An intriguing feature of PRDM9 is the exceptionally rapid evolution of its DNA-binding domain, which causes enormous variability in the locations of recombination events, even within a single species. To date, our understanding of PRDM9’s genome-wide distribution is based largely on its predicted DNA-binding specificity, however, the presence of a predicted consensus motif alone is not a strong predictor of recombination locations. Here, I will test the hypothesis that additional factors act in concert with the rapidly evolving PRDM9 protein to specify sites of meiotic recombination events. I will directly map and compare the genome-wide distribution of PRDM9 in different mouse strains to understand the evolution and principles of its binding. I will also pioneer a single-cell based approach that can distinguish between different classes of recombination events to build a high-resolution genome-wide recombination map to further dissect the role of PRDM9, and to identify additional factors, that shape the recombination landscape. Fields of science natural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological scienceszoologymammalogynatural sciencesbiological sciencesgeneticshereditynatural sciencesbiological sciencesgeneticsgenomes Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Topic(s) MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF) Call for proposal H2020-MSCA-IF-2014 See other projects for this call Funding Scheme MSCA-IF-EF-ST - Standard EF Coordinator THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE Net EU contribution € 195 454,80 Address TRINITY LANE THE OLD SCHOOLS CB2 1TN Cambridge United Kingdom See on map Region East of England East Anglia Cambridgeshire CC Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 195 454,80
