Cel The regulated diffusion of light-harvesting complexes in photosynthetic membranes is essential for photosynthesis, for instance for the redistribution of light-harvesting complexes between Photosystems I and II. To date, however, little is understood about the mobility of these proteins through the highly crowded membrane environment. In contrast to the huge amount of structural research that has been performed on biomolecules in the past, research on the dynamics is lagging far behind. The major limitation is the fact that the nanometer size regime of biomolecules is not accessible by classical optical techniques owing to the diffraction limit of light. To overcome this problem, we propose to use plasmonic nanoantennas, which are able to confine light at the nanoscale. In combination with Fluorescence Correlation Spectroscopy (FCS) this will allow measuring protein mobility in photosynthetic membranes with high, tens of nanometers, resolution. Directional and functional protein movement will be induced by illumination of the chloroplasts using specific light conditions; simultaneously following the protein movement will give new insights in how plants optimize their light harvesting capacity under fluctuating light conditions. The new nanoantenna-FCS based method, which we will develop to measure the diffusion of membrane proteins with nanometer resolution, has the potential to become widely used for nanobioimaging and ultrasensitive biosensing.This research will be performed by Dr. E Wientjes in the Laboratory of Biophysics of Wageningen University led by photosynthesis expert Prof. H van Amerongen. A long-term collaboration will be started with Wientjes current supervisor Prof. NF van Hulst and Prof. MF Garcia-Parajo from the Institute of Photonic Sciences in Barcelona who are leading scientists in nanophotonics. The outcome of this project will provide biophysicists with a new method to study membrane protein dynamics with super resolution. Dziedzina nauki natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesphysical sciencesopticsmicroscopyfluorescence lifetime imagingnatural sciencesbiological sciencesbotanynatural sciencesphysical sciencestheoretical physicsparticle physicsphotonsnatural sciencesphysical sciencesopticsspectroscopy Słowa kluczowe photosynthesis membrane fluorescence correlation spectroscopy nanoantenna plasmonics diffusion Program(-y) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Temat(-y) MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF) Zaproszenie do składania wniosków H2020-MSCA-IF-2014 Zobacz inne projekty w ramach tego zaproszenia System finansowania MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Koordynator WAGENINGEN UNIVERSITY Wkład UE netto € 177 598,80 Adres DROEVENDAALSESTEEG 4 6708 PB Wageningen Niderlandy Zobacz na mapie Region Oost-Nederland Gelderland Veluwe Rodzaj działalności Higher or Secondary Education Establishments Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Uczestnictwo w unijnych programach w zakresie badań i innowacji Opens in new window sieć współpracy HORIZON Opens in new window Koszt całkowity € 177 598,80
