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Cloning and functional characterization of a complex resistance locus from ‘Geneva’ to breed apple cultivars with durable scab resistance

Objective

Apple scab caused by Venturia inaequalis is the major constraint to apple production worldwide, causing severe economic losses. As current commercial cultivars are highly susceptible to scab, introduction of new scab-resistant cultivars will reduce the intensive use of pesticides now required to control this disease. Although the ‘Geneva’ apple is an important source of resistance for breeding, its complex scab resistance has not been properly characterized. In preliminary studies, we mapped to chromosome 4 of ‘Geneva’ a 5 cM region containing three genes conferring both dominant and recessive scab resistance, which corresponds to a 2 Mbp region containing nine candidate NBS-LRR resistance genes on the physical map of ‘Golden Delicious’ (GD). This provided the first evidence of recessive genetic control of apple scab resistance. In this project proposal, we will further characterize this complex locus, employing next generation sequencing, together with bioinformatics and functional analysis of disease candidate resistance genes (CRGs): (1) we will sequence the resistance locus in ‘Geneva’ and identify CRGs that are polymorphic (presence/absence, or sequence polymorphism) between the resistant ‘Geneva’ and the susceptible GD; (2) we will clone each CRG with its native promoter, terminator and introns; and (3) transform susceptible lines with the individual CRGs to evaluate their effect on the level of disease resistance and its race-specific spectrum. This will not only build a better understanding of the genetic basis of apple scab resistance and the gene-for-gene relationships between the pathogen and the host, but it will enable the development of molecular markers for breeding new ‘sprayfree’ cultivars with durable scab resistance.

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Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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Call for proposal

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(opens in new window) H2020-MSCA-IF-2014

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Coordinator

FONDAZIONE EDMUND MACH
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 238 354,20
Address
VIA EDMONDO MACH 1
38098 San Michele All'Adige
Italy

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Region
Nord-Est Provincia Autonoma di Trento Trento
Activity type
Higher or Secondary Education Establishments
Links
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 238 354,20

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