Imaging nucleic acid metabolism in cells

Projektinformationen

INAME

ID Finanzhilfevereinbarung: 656872

DOI 10.3030/656872

Abgeschlossenes Projekt

EK-Unterschriftsdatum 13 April 2015

Startdatum 4 Januar 2016

Enddatum 3 Januar 2018

Finanziert unter

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Gesamtkosten € 195 454,80

EU-Beitrag € 195 454,80

Koordiniert durch

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
United Kingdom

Ziel

In this project I, Pawan Kumar, a Nucleic Acid Chemist seek to team up with a pioneer in Nucleic Acid Chemistry and Biology (Professor Tom Brown) to carry out a highly interdisciplinary study at the University of Oxford to obtain deeper insights into DNA and RNA synthesis and metabolism in living cells and its application to cancer research. I propose to detect and quantify nucleic acid synthesis in proliferating cells by fluorescence without the requirement for toxic metal ions or antibodies. Earlier known methods such as [3H]thymidine and 5-bromo-2'-deoxyuridine labelling are either slow and labour intensive or require the use of harsh conditions. Incorporation of 5-ethynyl-2'-deoxyuridine into newly synthesized DNA and its subsequent detection with an azide derivative of a fluorescent dye under copper catalyzed alkyne azide cycloaddition (CuAAC) reaction conditions presents a better alternative. However, cytotoxicity of copper salts restricts its use for living cells. I will use the strain promoted alkyne azide cycloaddition reaction, Diels-Alder reaction, and inverse electron demand Diels-Alder reaction to study cellular DNA and RNA. None of these reactions require the use of toxic metal salts. I will develop the conditions under which both DNA and RNA will be stable, so that it will be possible to isolate intact fluorescent nucleic acids from cells for detailed analysis. I will prepare the modified nucleosides and use them to label newly synthesized DNA and RNA in cells enabling their detection by reaction with fluorophores by using metal free click ligation reactions. The study will provide a better understanding of the mechanisms regulating DNA replication and the interplay between transcription and DNA replication. In the project I will develop techniques to provide information on the toxic effect of antimetabolites used commonly in anti-cancer therapies, and for identifying the mechanisms of viral replication and understanding the viral life cycle.

Wissenschaftliches Gebiet

• medical and health sciences › basic medicine › pharmacology and pharmacy › drug discovery
• natural sciences › biological sciences › biochemistry › biomolecules › nucleic acids
• medical and health sciences › basic medicine › neurology › muscular dystrophies
• natural sciences › biological sciences › genetics › DNA
• natural sciences › biological sciences › genetics › RNA

Programm/Programme

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility

Thema/Themen

• MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF)

Aufforderung zur Vorschlagseinreichung

H2020-MSCA-IF-2014

Finanzierungsplan

MSCA-IF-EF-ST - Standard EF

Koordinator

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD

• Netto-EU-Beitrag: € 195 454,80
• Adresse: WELLINGTON SQUARE UNIVERSITY OFFICES ; OX1 2JD Oxford ; Vereinigtes Königreich
• Region: South East (England) › Berkshire, Buckinghamshire and Oxfordshire › Oxfordshire
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: € 195 454,80