Objective
The research field of proteomics proceeds rapidly thanks to recent technological advances of instrumentation, methodology,
and software development. Next Generation Proteomics (NGP) refers to the integration of these three areas to provide a
systematic approach for measuring proteome dynamics in both time and space during various cellular responses. In this
proposal, I will analyze pre-ribosomal proteome dynamics in response to intracellular energy status using a NGP approach.
Specifically, I will determine how cancer cells regulate Ribosome Biogenesis (RiBi) to aid their survival under conditions of
energy deprivation, which frequently occurs in connection with solid tumour development. The host laboratory is well known
for developing and applying NGP strategies and will provide me with training and access to all of the equipment and
resources required. To carry out this project I will first optimise methods for purification of human pre-ribosomal particles,
combining my existing knowledge of RiBi with expertise from the Lamond group in nucleolar isolation. I will then use a
quantitative proteomics approach to analyze pre-ribosomes isolated from cells grown under conditions of varied glucose
deprivation. I will compare the components of pre-40S, pre-60S and pre-90S particles, respectively, using SILAC and the
PepTracker software developed in the Lamond group. This project is based on my recent data showing that 47S pre-rRNA
processing, which occurs in pre-90S particles, is suppressed by glucose deprivation in human adenocarcinoma HeLa and
MCF7 cell lines. As the proteins contained in human pre-ribosomes are less well characterised than the corresponding
yeast proteins, I will incorporate these results in a searchable database of human RiBi factors that will be freely available to
the community. As RiBi is the most energy-consuming process in eukaryotic cell the results of this project may lead to novel
cancer treatment strategies which target deregulation of RiBi.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences physical sciences optics microscopy electron microscopy
- natural sciences computer and information sciences software software development
- natural sciences biological sciences genetics RNA
- natural sciences chemical sciences analytical chemistry mass spectrometry
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2014
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
DD1 4HN Dundee
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.