Objective
Active mRNA transport and localisation are crucial for spatiotemporal control of gene expression. In neurons, dendritic mRNA transport and local translational control are required for synaptic plasticity and memory formation. Misregulation has been linked to severe diseases. RNA-binding proteins (RBPs), kinesin motor proteins, mRNAs and noncoding RNAs (ncRNAs) are involved in dendritic mRNA transport and translational regulation. Still it is not known which essential set of factors is required to enable kinesin-dependent mRNA transport. Further, many RBPs required for mRNA transport simultaneously act as regulators of translation. How these crucial activities are mechanistically linked is unsolved. To anwswer these questions, this project aims to reveal essential mechanisms by two independent biochemical in vitro reconstitution approaches: (1) I will reconstitute kinesin-dependent transport of a dendritically localised mRNA and noncoding RNA using candidate proteins and RNAs from literature and an unpublished result of the host laboratory. (2) I will analyse the dual function of RBPs in mRNA transport and translational regulation. To this end, a microfluidics-coupled in vitro translation imaging assay (TIA) will be developed to study the effect of RBPs and ncRNAs on translation in real-time. Finally, the in vitro motility assay will be combined with the TIA to investigate the impact of translational regulation on mRNA transport. Using these techniques I will address key questions: (i) Which are the essential components required for kinesin-dependent mRNA transport? (ii) How are mRNA transport and translational regulation mechanistically connected? This project aims to unravel fundamental principles, improving our knowledge of neuronal mRNA transport and gene expression on a mechanistic level. Additionally, the experimental framework developed herein will enable the field to answer further important questions relating to the growing field of localised translation.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences neurobiology
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences developmental biology
- natural sciences biological sciences genetics mutation
- natural sciences biological sciences genetics RNA
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2014
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
08003 Barcelona
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.