Objective
An ambitious and multidisciplinary project is proposed to identify on the large scale the molecular targets of different chemical forms of nickel in some human cells. For this aim, a comprehensive and multidisciplinary program is proposed, including toxicity assays, non-denaturing procedures and the development of analytical schemes based on multidimensional electrophoresis and chromatographic separation.
Despite nickel is classified as carcinogenic and nickel allergy is common worldwide, the molecular mechanisms responsible of nickel toxicity are not know. In this sense, to determine the carcinogenicity potential of nickel compounds, the information about the molecular targets of nickel is critical. There is an urgent need to obtain information about the molecular target depending on the chemical form of nickel, as they are not known yet. This project will study a broad range of different chemical forms of nickel in two selected human cells. Cells will be incubated with the different chemical forms in different conditions and the cytotoxicity will be measured. In order to isolate the native nickel complexes, an optimal non-denaturing procedure of will be developed. Information about the uptake of nickel to cells will be obtained and correlated with the citotoxicity results. Lastly, the identification of the molecular targets will be carried out through the development of novel analytical strategies based on the combination of different separation techniques with different detection techniques.
The great novelty of this project is based on the combination of the information obatined about nickel toxicity and nickel uptake with molecular nickel speciation. The information obtained in the project will help to future understanding of molecular mechanisms of nickel related to nickel allergy and nickel carcenogicity. Additionally, the understanding of these molecular mechanisms will also help to the understanding on the mechanisms of other metal allergies.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences software
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences chemical sciences inorganic chemistry transition metals
- natural sciences chemical sciences electrochemistry electrophoresis
- natural sciences chemical sciences analytical chemistry mass spectrometry
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2014
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
75794 PARIS
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.