Objective
Site-specific proteolytic processing is an irreversible post-translational protein modification that generates distinct protein species with new functions, interactions and subcellular locations. In plants, proteolytic processing regulates hormonal and stress signaling leading to adaptation of metabolic pathways and is implicated in plant-pathogen interactions. Despite their importance, proteolytic processes have largely been identified serendipitously, specific cleavage sites have rarely been identified and only a few of the hundreds of proteases encoded in plant genomes (>800 in Arabidopsis) have been linked to any substrates.
Positional proteomics enables system-wide identification of proteolytic processing and protease substrate repertoires through quantitative determination of protein N- or C-termini. ProPlantStress will employ these approaches, which I co-developed during my postdoctoral research, to two linked abiotic and biotic stress conditions: i) Time-resolved mapping of chloroplast protein processing induced by high intensity light will reveal novel mechanisms of retrograde signal transduction, stress response and acclimation; ii) Profiling of protein processing triggered by pathogen recognition, combined with substrate identification for selected host and bacterial pathogen effector proteases will identify proteins with novel functions in plant immune responses and systemic signaling.
Importantly, ProPlantStress will not merely catalogue termini and substrates: Mapping of cleavage sites to the protein domains and correlation with other modifications, such as phosphorylation, generates testable hypotheses on the function of processed protein species that will be examined in detail.
ProPlantStress will thereby provide fundamental insights into proteolytic mechanisms underlying plant stress responses that are unattainable by other means. In the long term such knowledge is needed to develop new strategies for crop protection and mitigation of harvest loss.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- agricultural sciences agriculture, forestry, and fisheries agriculture agronomy plant protection
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences chemical sciences analytical chemistry mass spectrometry
- natural sciences biological sciences genetics genomes
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
ERC-STG - Starting Grant
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Call for proposal
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(opens in new window) ERC-2014-STG
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52428 JULICH
Germany
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