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Development of Novel Analytical Methods to assess Nanoparticle cytotoxicity

Objective

Nanoparticles , NPs, are being used to produce novel materials with unique physico-chemical properties with many applications in fields such as medicine, energy and electronics, consumer goods, among others. Some studies have revealed that the same specific properties that make NPs so unique could also be responsible for potential harmful effects on the environment and human health. Currently, the assessment of their impact is hampered by limited analytical capabilities to detect them in complex natural matrices. New sensitive and selective analytical methods must be developed and validated in order to elucidate their toxicological effects prior to increasing and promoting the NPs production. Herein, new analytical methodologies based on inductively coupled plasma mass spectrometry (ICP-MS) are proposed to evaluate the toxicity of metallic NPs in cells, one of the most powerful and attractive model systems for toxicological assays. The proposed methodology consists on the use of laser ablation (LA) coupled to ICP-MS for spatially resolved bioimaging of the distribution of NPs in single cells upon different NPs incubation experiments; furthermore, a micro-droplet generator (µDG) for sample introduction of single cells into a sector field ICP-MS will be employed to improve the NPs detection sensitivity. The expected results will give essential insights into nanoparticle/cell interactions and will have implications for the development of analytical methods based on applications of nano-particles for medical diagnostics and therapeutics.

Fields of science (EuroSciVoc)

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Programme(s)

Multi-annual funding programmes that define the EU’s priorities for research and innovation.

Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MSCA-IF-EF-ST - Standard EF

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2014

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Coordinator

BUNDESANSTALT FUER MATERIALFORSCHUNG UND -PRUEFUNG
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 159 460,80
Address
Unter den Eichen 87
12205 Berlin
Germany

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Region
Berlin Berlin Berlin
Activity type
Research Organisations
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 159 460,80
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