Objectif Cellular studies in neuroscience are hampered by the fact that in vitro cultures of primary neurons are complex, variable and heterogeneous, making studies on cultured neurons time consuming, labour intensive and not scalable. Especially for single cell studies on synaptic transmission and cellular trafficking, this is a major limitation. Both in academia and industry, mitotic cells are commonly used as a proxy for neurons (e.g. PC12 cells). However, while being less variable and better scalable, such cells model neurons only to a limited extent. These cells make no functional contacts (synapses) and do not have the same polarized organization that neurons have, which limits the validity for studies on synaptic transmission and cellular trafficking. Most commonly prescribed drugs for brain disorders target synapses. Yet, drug screening, lead finding/optimization and safety studies build either on cells that make no synapses or on labor-intensive low-throughput assays. Hence, new, standardized, scalable assays using real (synaptic) neurons, will provide a substantial step forward both for academia and industry.This application aims to develop a nano-electrode chip with an electrode array to stimulate, record and image many single neurons with high-resolution, for a period up to several weeks. This approach provides imaging data at single synapse and single organelle resolution and synaptic transmission data at high-throughput. Together, this will not only facilitate and standardize cellular assays using real neurons, but also upscale throughput 100 fold. In addition to the chip itself, we also see market potential for the method (growing/recording neurons on chips) and for a fee-for-service company that offers high throughput compound screening on synaptic neurons. Champ scientifique natural sciencesbiological sciencesneurobiology Programme(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Thème(s) ERC-PoC-2014 - ERC Proof of Concept Grant Appel à propositions ERC-2014-PoC Voir d’autres projets de cet appel Régime de financement ERC-POC - Proof of Concept Grant Institution d’accueil STICHTING AMSTERDAM UMC Contribution nette de l'UE € 135 617,12 Adresse DE BOELELAAN 1117 1081 HV Amsterdam Pays-Bas Voir sur la carte Région West-Nederland Noord-Holland Groot-Amsterdam Type d’activité Research Organisations Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 135 617,12 Bénéficiaires (2) Trier par ordre alphabétique Trier par contribution nette de l'UE Tout développer Tout réduire STICHTING AMSTERDAM UMC Pays-Bas Contribution nette de l'UE € 135 617,12 Adresse DE BOELELAAN 1117 1081 HV Amsterdam Voir sur la carte Région West-Nederland Noord-Holland Groot-Amsterdam Type d’activité Research Organisations Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 135 617,12 STICHTING VU Participation terminée Pays-Bas Contribution nette de l'UE € 13 857,88 Adresse DE BOELELAAN 1105 1081 HV Amsterdam Voir sur la carte Région West-Nederland Noord-Holland Groot-Amsterdam Type d’activité Higher or Secondary Education Establishments Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 13 857,88