Cel Cellular studies in neuroscience are hampered by the fact that in vitro cultures of primary neurons are complex, variable and heterogeneous, making studies on cultured neurons time consuming, labour intensive and not scalable. Especially for single cell studies on synaptic transmission and cellular trafficking, this is a major limitation. Both in academia and industry, mitotic cells are commonly used as a proxy for neurons (e.g. PC12 cells). However, while being less variable and better scalable, such cells model neurons only to a limited extent. These cells make no functional contacts (synapses) and do not have the same polarized organization that neurons have, which limits the validity for studies on synaptic transmission and cellular trafficking. Most commonly prescribed drugs for brain disorders target synapses. Yet, drug screening, lead finding/optimization and safety studies build either on cells that make no synapses or on labor-intensive low-throughput assays. Hence, new, standardized, scalable assays using real (synaptic) neurons, will provide a substantial step forward both for academia and industry.This application aims to develop a nano-electrode chip with an electrode array to stimulate, record and image many single neurons with high-resolution, for a period up to several weeks. This approach provides imaging data at single synapse and single organelle resolution and synaptic transmission data at high-throughput. Together, this will not only facilitate and standardize cellular assays using real neurons, but also upscale throughput 100 fold. In addition to the chip itself, we also see market potential for the method (growing/recording neurons on chips) and for a fee-for-service company that offers high throughput compound screening on synaptic neurons. Dziedzina nauki natural sciencesbiological sciencesneurobiology Program(-y) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Temat(-y) ERC-PoC-2014 - ERC Proof of Concept Grant Zaproszenie do składania wniosków ERC-2014-PoC Zobacz inne projekty w ramach tego zaproszenia System finansowania ERC-POC - Proof of Concept Grant Instytucja przyjmująca STICHTING AMSTERDAM UMC Wkład UE netto € 135 617,12 Adres DE BOELELAAN 1117 1081 HV Amsterdam Niderlandy Zobacz na mapie Region West-Nederland Noord-Holland Groot-Amsterdam Rodzaj działalności Research Organisations Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Uczestnictwo w unijnych programach w zakresie badań i innowacji Opens in new window sieć współpracy HORIZON Opens in new window Koszt całkowity € 135 617,12 Beneficjenci (2) Sortuj alfabetycznie Sortuj według wkładu UE netto Rozwiń wszystko Zwiń wszystko STICHTING AMSTERDAM UMC Niderlandy Wkład UE netto € 135 617,12 Adres DE BOELELAAN 1117 1081 HV Amsterdam Zobacz na mapie Region West-Nederland Noord-Holland Groot-Amsterdam Rodzaj działalności Research Organisations Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Uczestnictwo w unijnych programach w zakresie badań i innowacji Opens in new window sieć współpracy HORIZON Opens in new window Koszt całkowity € 135 617,12 STICHTING VU Zakończenie uczestnictwa Niderlandy Wkład UE netto € 13 857,88 Adres DE BOELELAAN 1105 1081 HV Amsterdam Zobacz na mapie Region West-Nederland Noord-Holland Groot-Amsterdam Rodzaj działalności Higher or Secondary Education Establishments Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Uczestnictwo w unijnych programach w zakresie badań i innowacji Opens in new window sieć współpracy HORIZON Opens in new window Koszt całkowity € 13 857,88