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Investigating glial glycogen utilization for ion homeostasis in the brain and its relevance to epileptogenesis: electrophysiology and pharmacology in awake behaving mice

Objective

We do not yet fully understand the cellular basis of brain energy metabolism. The high energy consumption of mammalian brain sets information processing under critical metabolic constraints. Energy efficiency in brain signaling is supported by functional and metabolic interactions between neuronal and astrocytic cells. Specifically, during neuronal activity astrocytes rapidly take up neuronally-released compounds from the extracellular space, including potassium (K+) and transmitter molecules. These operations affect brain excitability and their dysfunction can increase susceptibility to seizures and eventually lead to epilepsy. Importantly, ion homeostasis in astrocytes is fueled by astrocytic glycogen, the sole cerebral energy store. The primary aim of the present project is to investigate how metabolism of glycogen in astrocytes supports and influences the different stages of neuronal activity under normal and epileptogenic conditions. I hypothesize that K+-induced glycogenolysis in astrocytes controls neuronal excitability (functional role) as well as neuronal glucose uptake (metabolic role). These ideas are supported by the recently demonstrated requirement of astrocytic glycogenolysis for the uptake of extracellular K+ obtained in cell cultures and by preliminary results that I obtained through kinetic analysis. The present project will tackle, for the first time in awake behaving mice, the characterization of activity-dependent brain glycogen metabolism by means of electrophysiological and pharmacological experiments. The outcomes will provide essential insights into the mechanisms underlying normal ion homeostasis and its impairment in epilepsy as well as other pathologies related to aberrations in brain energy metabolism. The project will have a substantial impact on my career, as new skills in invasive experimental techniques on awake animals will complement my previous expertise in non-invasive functional magnetic resonance methods on human subjects.

Fields of science (EuroSciVoc)

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Topic(s)

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Funding Scheme

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MSCA-IF-EF-ST - Standard EF

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Call for proposal

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(opens in new window) H2020-MSCA-IF-2015

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Coordinator

KOBENHAVNS UNIVERSITET
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 212 194,80
Address
NORREGADE 10
1165 KOBENHAVN
Denmark

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Region
Danmark Hovedstaden Byen København
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 212 194,80
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