Objective
Duplication of the genome and its division into two daughter cells during mitosis is vital for survival of the organism. Cells have multiple mechanisms to ensure that this process is accomplished correctly thereby preserving the integrity of the genome. The final check before cell division is made by the NoCut abscission pathway. In yeast and animal cells, this mechanism monitors completion of chromosome separation, delaying abscission when chromosome bridges spanning the division site are detected.
Aurora B is essential for NoCut function, and several of its targets in this pathway have been identified. In budding yeast, NoCut can be triggered by bridges caused by defects in chromosome condensation, decatenation and replication but importantly not by dicentric chromosome bridges. This suggests that structural features of chromatin bridges are essential to generate the NoCut signal. We will investigate the molecular basis of this differential bridge recognition and the signalling pathway acting upstream of Aurora B.
We will define the composition of fine and ultra-fine chromatin bridges during cytokinesis in human cells at unprecedented resolution by super-resolution microscopy using dSTORM imaging. In parallel, we will use budding yeast to investigate the role of DNA binding proteins as sensors in the NoCut pathway. We will then establish the significance of these findings in human cells, by assaying the function of putative homologs in NoCut, and their localization in chromatin bridges by dSTORM. By combining approaches in two model systems we will define both the molecular and physical constrains for NoCut activation upstream of the established components of the NoCut pathway.
Chromosome instability is associated with many human tumours and in some cases with advanced disease making the detailed characterization of this pathway relevant in our understanding of both basic cellular processes and human disease.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences physical sciences optics microscopy super resolution microscopy
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics chromosomes
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2015
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
08003 Barcelona
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.