Objectif The main goal of this project is to understand deep-sea prokaryotic activity and metabolism under in situ hydrostatic pressure and reveal the contribution of piezophilic prokaryotes to the total prokaryotic community in the dark ocean. In the project, deep-sea prokaryotes will be incubated under in situ hydrostatic pressure with an in situ microbial incubator (ISMI) which has been developed in the lab of the applicant’s PhD supervisor in Japan. The ISMI, as the name suggests, is an instrument to incubate deep-sea biota at the depth of sampling and hence, without change of the hydrostatic pressure and temperature. Deep-sea prokaryotes will also be incubated under atmospheric pressure at otherwise identical conditions as a control, thus, this approach will reveal the impact of hydrostatic pressure on prokaryotic activity. Consequently, we will obtain information on the phylogeny and metabolism of piezophilic and piezotolerant prokaryotic populations in the dark ocean. The ISMI will be deployed during two long (ca. 1 month) research cruises, and a short (a week) cruise in the project. Prokaryotic heterotrophic and autotrophic production under in situ hydrostatic pressure will be measured, and piezophilic, piezotolerant, and piezosensitive prokaryotes will be studied with microautoradiography combined with catalyzed reporter deposition fluorescence in situ hybridization (MICRO-CARD-FISH) and omics approaches. Measuring metabolic activity of deep-sea microbes under in situ pressure conditions represents a major endeavor, and it remains unknown how large the fraction of piezophilic and piezotolerant prokaryotes in the deep ocean really is. The project has the potential to provide an answer to the question of the metabolic activity of microbes under in situ pressure conditions in the global ocean. Champ scientifique natural sciencesbiological sciencesbiological morphologycomparative morphologynatural sciencesbiological sciencesmicrobiologybacteriologynatural scienceschemical sciencesinorganic chemistryinorganic compoundsnatural sciencesphysical sciencesclassical mechanicsfluid mechanicsfluid staticsnatural sciencesearth and related environmental sciencesatmospheric sciencesmeteorologyatmospheric pressure Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Thème(s) MSCA-IF-2015-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF) Appel à propositions H2020-MSCA-IF-2015 Voir d’autres projets de cet appel Régime de financement MSCA-IF-EF-ST - Standard EF Coordinateur UNIVERSITAT WIEN Contribution nette de l'UE € 178 156,80 Adresse UNIVERSITATSRING 1 1010 Wien Autriche Voir sur la carte Région Ostösterreich Wien Wien Type d’activité Higher or Secondary Education Establishments Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 178 156,80
