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Development and Characterisation of New Immunogenic GEMMs of Lung Cancer

Objective

Many studies have recently archived a remarkable clinical efficacy of immunotherapeutic treatments in several cancers including non-small cell lung cancer (NSCLC). However, these approaches are not always successful and it is currently impossible to predict a therapeutic response or serious adverse event. These fundamental issues need to be addressed and, to recapitulate the complexity of interactions between immune cells and cancer, this has to be done in vivo. However, genetically engineered mouse models (GEMMs) of NSCLC do not mirror the genomic instability and the high mutations rate of their human counterparts. Consequently, these tumours rarely present neo-antigens that can induce an immune response, and are considered poorly immunogenic. To our knowledge, a genetically defined model of an immunogenic spontaneous tumour does not exist. Here, we propose to develop such an immunogenic GEMM (iGEMM).
The leading mouse model of lung cancer is the GEMM Kraslsl-G12D/wtTrp53fl/fl (KP). We propose two strategies to induce either genomic instability or mutation rate in the KP model. 1) We will overexpress Mad2, a component of the mitotic spindle assembly checkpoint, to induce aneuploidy in KP tumours; 2) We will overexpress the cytidine deaminase Apobec3 to increase the mutation rate of KP tumours.
The resulting immunogenic GEMMs (iGEMMs) will be characterised and used to assess new therapies that trigger an immune response and to address mechanisms which drive immunotolerance. iGEMMs will facilitate the investigation of currently available immunotherapies which will help predict clinical response to these therapies and investigation on alternative strategies to treat resistant lung tumours. All together, these investigations will provide novel information regarding the best strategies to cure NSCLC by modulation of the IS.

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Programme(s)

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Topic(s)

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Funding Scheme

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MSCA-IF-EF-ST - Standard EF

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Call for proposal

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(opens in new window) H2020-MSCA-IF-2015

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Coordinator

THE FRANCIS CRICK INSTITUTE LIMITED
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 183 454,80
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1 MIDLAND ROAD
NW1 1AT London
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Region
London Inner London — West Camden and City of London
Activity type
Research Organisations
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 183 454,80
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