Objectif The mammalian brain integrates behaviorally relevant sensory information by recruiting large parts of the neocortex to enable precise perception, apt decisions and adequate actions. The large-scale interactions and the distinct roles of the various neocortical regions, namely frontal motor-related areas and posterior sensory-related regions, remain poorly understood. Here, we aim to characterize how behavior-related activity in higher-order regions (e.g. frontal area) of the mouse is spatiotemporally organized, and how does it relate to lower-order regions (e.g. somatosensory cortex). Mice will be trained on an S1-dependent texture discrimination task under head-fixed conditions, enabling simultaneous imaging of neuronal populations. First, we will use a novel wide-field preparation to map lower and higher-order functions throughout the cortex. Next, we will zoom-in on specific areas of interest using two-photon microscopy to achieve single cell resolution. Finally, we will use an array of labeling techniques to track behavior-dependent activity of neuronal populations that project to a specific area. A special emphasis will be to identify how the activity in subsets of neurons that project ‘top-down’, from frontal areas to the primary sensory area, relates to different behavioral aspects. We expect that our results will provide fundamental insights into the contribution of frontal cortical regions to perception-related and decision-related activity before sensation (anticipation), during sensation (texture touch) and after sensation (holding in memory and 'licking for reward' action). We believe that this multidisciplinary project in behaving animals is a challenging and promising approach bound to generate novel and exciting results which will be of great interest to the scientific community. Champ scientifique natural sciencescomputer and information sciencesdata sciencenatural sciencesbiological sciencesneurobiologycognitive neurosciencemedical and health sciencesbasic medicineneurologydementiaalzheimernatural sciencesphysical sciencesopticsmicroscopysocial sciencessociologysocial issuessocial inequalities Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Thème(s) MSCA-IF-2014-GF - Marie Skłodowska-Curie Individual Fellowships (IF-GF) Appel à propositions H2020-MSCA-IF-2014 Voir d’autres projets de cet appel Régime de financement MSCA-IF-GF - Global Fellowships Coordinateur THE HEBREW UNIVERSITY OF JERUSALEM Contribution nette de l'UE € 278 674,20 Adresse EDMOND J SAFRA CAMPUS GIVAT RAM 91904 Jerusalem Israël Voir sur la carte Type d’activité Higher or Secondary Education Establishments Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 278 674,20 Partenaires (1) Trier par ordre alphabétique Trier par contribution nette de l'UE Tout développer Tout réduire Partenaire Les organisations partenaires contribuent à la mise en œuvre de l’action, mais ne signent pas la convention de subvention. UNIVERSITAT ZURICH Suisse Contribution nette de l'UE € 0,00 Adresse RAMISTRASSE 71 8006 Zurich Voir sur la carte Région Schweiz/Suisse/Svizzera Zürich Zürich Type d’activité Higher or Secondary Education Establishments Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 187 419,60