Microfluidic-assisted fabrication of artifical microniches for bone marrow stem cells

Projektinformationen

MicroNICHE

ID Finanzhilfevereinbarung: 705163

DOI 10.3030/705163

Abgeschlossenes Projekt

EK-Unterschriftsdatum 4 April 2016

Startdatum 1 Juli 2016

Enddatum 26 Februar 2020

Finanziert unter

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Gesamtkosten € 239 191,21

EU-Beitrag € 239 191,20

Koordiniert durch

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain

Ziel

There is a growing interest in adult stem cells, especially from bone marrow, for regenerative medicine. Hematopoietic stem cells, a type of bone marrow stem cells, alone cannot be expanded in vitro; in vivo, they reside in a microenvironment known as a niche that maintains them in a quiescent state until prompted to differentiate. The stem cell niche provides structural and trophic support and the appropriate homeostasis to regulate stem cell function. Additionally to regulatory factors in these stem cell niches, a number of environmental and mechanical signals arising from the extracellular matrix are crucial regulators of stem cell fate. In order to expedite for basic studies of bone marrow stem cells, and further translational implementation, any realistic approach to the native stem cell niche requires: to engineer a biomimetic 3D-microenvironment, and then to develop artificial microniches with the key functional features reconstructed. High-throughput microfluidic technology offers high promise, however, adaptation to accommodate adult stem cells in artificially fabricated niches remains still a challenge. Microfluidic-assisted culture systems should not only allow maintaining cell homeostasis through biochemical and mechanical stimulation, but also modulating adult stem cell renewal and differentiation through microscale patterning of cells and extracellular materials in biomimetic microniches. This project aims at the microfluidic reconstruction of an artificial stem cell niches. In this proof-of-concept, a bone marrow stem cell microniche with tunable size, material and topography will be developed by integrating novel fabrication microfluidics with material engineering.

Wissenschaftliches Gebiet

• natural sciences › physical sciences › classical mechanics › fluid mechanics › microfluidics
• natural sciences › physical sciences › astronomy › planetary sciences › planetary geology
• medical and health sciences › medical biotechnology › cells technologies › stem cells
• medical and health sciences › basic medicine › physiology › homeostasis

Programm/Programme

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility

Thema/Themen

• MSCA-IF-2015-GF - Marie Skłodowska-Curie Individual Fellowships (IF-GF)

Aufforderung zur Vorschlagseinreichung

H2020-MSCA-IF-2015

Finanzierungsplan

MSCA-IF-GF - Global Fellowships

Koordinator

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

• Netto-EU-Beitrag: € 199 235,84
• Adresse: CALLE SERRANO 117 ; 28006 Madrid ; Spanien
• Region: Comunidad de Madrid › Comunidad de Madrid › Madrid
• Aktivitätstyp: Research Organisations
• Gesamtkosten: € 199 235,85

Beteiligte (1)

UNIVERSIDAD COMPLUTENSE DE MADRID

Beteiligung beendet

Spanien

• Netto-EU-Beitrag: € 39 955,36
• Adresse: AVENIDA DE SENECA 2 ; 28040 Madrid
• Region: Comunidad de Madrid › Comunidad de Madrid › Madrid
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: € 39 955,36

Partner (1)

Partner

Partnerorganisationen tragen zur Umsetzung der Aktion bei, unterzeichnen jedoch nicht die Finanzhilfevereinbarung.

TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

Vereinigte Staaten

• Netto-EU-Beitrag: € 0,00
• Adresse: AMSTERDAM AVENUE 1210 ROOM ; 10027 7003 New York
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: € 160 130,40