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Microfluidic-assisted fabrication of artifical microniches for bone marrow stem cells

Objective

There is a growing interest in adult stem cells, especially from bone marrow, for regenerative medicine. Hematopoietic stem cells, a type of bone marrow stem cells, alone cannot be expanded in vitro; in vivo, they reside in a microenvironment known as a niche that maintains them in a quiescent state until prompted to differentiate. The stem cell niche provides structural and trophic support and the appropriate homeostasis to regulate stem cell function. Additionally to regulatory factors in these stem cell niches, a number of environmental and mechanical signals arising from the extracellular matrix are crucial regulators of stem cell fate. In order to expedite for basic studies of bone marrow stem cells, and further translational implementation, any realistic approach to the native stem cell niche requires: to engineer a biomimetic 3D-microenvironment, and then to develop artificial microniches with the key functional features reconstructed. High-throughput microfluidic technology offers high promise, however, adaptation to accommodate adult stem cells in artificially fabricated niches remains still a challenge. Microfluidic-assisted culture systems should not only allow maintaining cell homeostasis through biochemical and mechanical stimulation, but also modulating adult stem cell renewal and differentiation through microscale patterning of cells and extracellular materials in biomimetic microniches. This project aims at the microfluidic reconstruction of an artificial stem cell niches. In this proof-of-concept, a bone marrow stem cell microniche with tunable size, material and topography will be developed by integrating novel fabrication microfluidics with material engineering.

Fields of science (EuroSciVoc)

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Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MSCA-IF-GF - Global Fellowships

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2015

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Coordinator

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 199 235,84
Address
CALLE SERRANO 117
28006 MADRID
Spain

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Region
Comunidad de Madrid Comunidad de Madrid Madrid
Activity type
Research Organisations
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 199 235,85

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Partners (1)

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