Single molecule imaging of transmembrane protein structure and function in their native state

Projektinformationen

TransPhorm

ID Finanzhilfevereinbarung: 683108

DOI 10.3030/683108

Projekt abgeschlossen

EK-Unterschriftsdatum 3 Juni 2016

Startdatum 1 September 2016

Enddatum 30 November 2022

Finanziert unter

• EXCELLENT SCIENCE - European Research Council (ERC)

Gesamtkosten € 2 434 650,00

EU-Beitrag € 2 434 650,00

Koordiniert durch

THE UNIVERSITY OF NOTTINGHAM
United Kingdom

Ziel

TransPhorm will pioneer a transformative technology platform based on Nitrogen Vacancy (NV) magnetometry to enable the structure and function of transmembrane proteins (TMPs) to be studied in their native state with unprecedented sensitivity and resolution. TMPs reside in the membrane of biological cells and are critical to cellular function and communication. It is essential that TMPs are characterised in their native state as their structure and function is dependent on their interaction with the local environment. This is technically demanding and despite previous attempts using a multitude of complementary techniques no single method has provided a suitable solution. Here a breakthrough approach will be taken to demonstrate in situ TMP characterisation with single molecule sensitivity, nanoscale spatial resolution and millisecond measurement speed.

The concepts proposed in TransPhorm are distinct from current implementations of NV magnetometry for detection and mapping of weak magnetic fields originating from external nuclear spins. Here magnetic field mapping will be achieved using a totally new approach based on widefield, high speed structured illumination total internal reflection microscopy. The concepts TransPhorm are built on will also enable structural and functional single molecular characterisation with high specificity by exploiting the outstanding sensitivity to the local environment of fluorine-19 Nuclear Magnetic Resonance (NMR) reporters and the ion selectivity of sodium-23 and potassium-39 NMR spectroscopy.

In short, TransPhorm will deliver a ground-breaking technology to far surpass current state-of-the-art techniques and provide the extreme sensitivity needed to understand the molecular scale dynamic changes that underpin TMP function. Overall the strategy and technologies proposed here will pave an untravelled path to the realisation of nanoscale NMR imaging and deliver tremendous scientific gains.

Wissenschaftliches Gebiet

• natural sciences › biological sciences › biochemistry › biomolecules › proteins
• natural sciences › physical sciences › optics › microscopy
• natural sciences › physical sciences › optics › spectroscopy › absorption spectroscopy

Schlüsselbegriffe

• TransPhorm

Programm/Programme

• H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme

Thema/Themen

• ERC-CoG-2015 - ERC Consolidator Grant

Aufforderung zur Vorschlagseinreichung

ERC-2015-CoG

Finanzierungsplan

ERC-COG - Consolidator Grant

Gastgebende Einrichtung

THE UNIVERSITY OF NOTTINGHAM

• Netto-EU-Beitrag: € 1 685 360,55
• Adresse: University Park ; NG7 2RD Nottingham ; Vereinigtes Königreich
• Region: East Midlands (England) › Derbyshire and Nottinghamshire › Nottingham
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: € 1 685 360,55

Begünstigte (2)

THE UNIVERSITY OF NOTTINGHAM

Vereinigtes Königreich

• Netto-EU-Beitrag: € 1 685 360,55
• Adresse: University Park ; NG7 2RD Nottingham
• Region: East Midlands (England) › Derbyshire and Nottinghamshire › Nottingham
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: € 1 685 360,55

UNIVERSITY OF KEELE ROYAL CHARTER

Beteiligung beendet

Vereinigtes Königreich

• Netto-EU-Beitrag: € 749 289,45
• Adresse: KEELE UNIVERSITY FINANCE DPT ; ST5 5BG Keele
• Region: West Midlands (England) › Shropshire and Staffordshire › Staffordshire CC
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: € 749 289,45