Objective
Identification of the molecular mechanisms by which gene regulatory landscapes integrate diverse signaling inputs into a robust and dynamic transcriptional output is key to a comprehensive mechanistic understanding of the systems that govern vertebrate organogenesis. We will tackle this by taking advantage of our in-depth knowledge of the self-regulatory signaling systems controlling vertebrate limb development. To capture the dynamics by which the major signaling pathways (BMP, SHH, WNT, FGF) interact to control gene expression, we will profile chromatin architecture, epigenetic marks and the interaction kinetics of transcriptional effectors with cis-regulatory modules (CRMs) during mouse limb bud development. First, we will focus on the Grem1 gene regulatory landscape, as this BMP antagonist is a key node in the system and its spatio-temporal expression is regulated by all four pathways. This analysis will capture the temporal activity of all CRMs in the Grem1 landscape and reveal the ones that are targets of one or several signaling pathways. The functional requirements of single and multiple CRMs with redundant functions will be assessed by gain- and loss-of-function genetics in mice. This analysis together with in silico simulations of the relevant interactions aims to reveal the underlying regulatory logic. Second, the genome-wide datasets will be used to analyse the gene regulatory landscapes of signals involved in epithelial-mesenchymal and auto-regulatory feedbacks. Third, the trans-cis regulatory alterations underlying the evolutionary diversifications that resulted in digit loss and reductions will be functionally studied by comparative analysis of mouse, bovine, pig and chicken limb buds. Finally, as aberrant Grem1 expression predisposes to various diseases and cancers, we will study the trans-cis regulatory alterations that affect the Grem1 landscape in a mouse model of SHH-induced cerebellar medulloblastomas, which depends on aberrant Grem1 expression.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences developmental biology
- natural sciences biological sciences genetics mutation
- natural sciences mathematics pure mathematics mathematical analysis functional analysis
- natural sciences biological sciences zoology mammalogy
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
ERC-ADG - Advanced Grant
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2015-AdG
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
4051 Basel
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.