Objective
As a result of the rapid development of next generation sequencing, we now have access to hundreds and often many thousands of sequences which belong to the same family. Such a large amount of sequence data for a particular protein family, along with recent developments in computational statistics, enables an entirely new kind of evolutionary analysis to be performed on sequences, where for the first time we can compute statistically significant networks of correlated mutations. The proposal describes an integrated programme of work to fully explore the potential applications of the new amino acid covariation techniques in predicting aspects of protein structure and function. A particular emphasis in this proposal are proteins which are difficult to study by experimental techniques i.e. disordered proteins, transmembrane proteins and large complexes. The first objective will be to explore key developments in the underpinning algorithms, tackling both the issue of needing very large numbers of homologous sequences and also the downstream 3-D embedding to produce viable models. The second objective will involve experimental work with a collaborator where the idea that de novo protein design techniques might be exploited to artificially expand the set of available sequences for a given proto-family will be explored. The third objective will focus specifically on transmembrane protein modelling, where covariation-based approaches have proven to be highly effective. Here the goal will be to extend our existing FILM3 method to encompass both beta-barrel type TM proteins, but also to try to handle the issue of homomultimers, which is a critical aspect of TM protein modelling as so many families are known to adopt higher orders of structure than the fold level alone. Finally, applications of covariation analysis to probing multiple conformations of disordered proteins will be developed, with a specific focus on interactions of disordered proteins with DNA and RNA.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences biochemistry biomolecules nucleic acids
- natural sciences computer and information sciences artificial intelligence machine learning deep learning
- natural sciences biological sciences genetics RNA
- natural sciences chemical sciences organic chemistry amines
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
ERC-ADG - Advanced Grant
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2015-AdG
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
WC1E 6BT LONDON
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.