Objective
The project 3D-Plant2Cells proposes to explore the impact of the agricultural mode on crops grown for human consumption. We are going to develop innovative interdisciplinary approaches to study the metabolome and the microbiota of plants in three-dimension (3D) at three different scales: the whole plant, the fruit, and the cellular scales. The objective of this study is to get a new insight on the metabolome and microbiota response to pesticide treatment. The hypothesis is that the chemistry and microbiome could change upon the use of conventional cultivation managements. To explore this hypothesis, two groups of plants will be cultivated in growth chamber and one will received pesticide treatments. Then, we will use an innovative interdisplinary approach to explore the entire pool of metabolite (metabolome) and the plant-associated commensal microbes (plant microbiota) on the whole plant surface (plant scale). Samples will be collected on the whole plant and their metabolite content will be profiled by high-resolution tandem-mass spectrometry (HR-MS/MS) and plant-associated micro-organism communities by amplicon sequence analysis. Results of these both inputs will be uploaded into a bioinformatic model representing a 3D topological map of the metabolome/microbiome over the entire organism surface. In a second part of the research project, we will study fruits of the cultivated specimens by 3D mass spectrometry imaging. The goals are to visualize, in a spatially-resolved way, the metabolome/microbiota response to pesticide treatments along with the pesticide distribution in 3D. The metabolome will be investigated at two scales: the fruit and the cell. The fruit scale will be explored by analyzing serial sections using matrix assisted laser desorption ionization (MALDI) or nanodesorption electrospray ionization (nanoDESI) .... The entire project will be an important contribution to assess the impact of pesticide on plant metabolome/microbiome and on food safety.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences computer and information sciences software
- natural sciences earth and related environmental sciences physical geography cartography
- agricultural sciences agriculture, forestry, and fisheries agriculture horticulture fruit growing
- natural sciences chemical sciences analytical chemistry mass spectrometry
- natural sciences biological sciences microbiology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2015
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
75794 PARIS
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.