Cel Efficient neuronal communication lies at the heart of all cognitive functions, and synaptic dysfunction underlies mental disorders such as autism. However, although over the past decades many components of synapses have been characterized, it is unknown how these constituents are assembled within synapses, and how this organization contributes to synapse function. The overall aim of this proposal is to understand how excitatory synapses are built to efficiently control neuronal function. Specifically, I aim to reveal the molecular organization that controls glutamate receptor positioning. While AMPA-type glutamate receptors concentrate in nano-domains within the synaptic core that directly apposes the presynaptic release site, metabotropic glutamate receptors accumulate in a distinct perisynaptic domain considerably further from the release site. Despite that this organization critically controls synaptic transmission and plasticity, we know little about the mechanisms that underlie the spatial and temporal segregation of glutamate receptor subtypes into these distinct subsynaptic domains. To address this, I developed single-molecule imaging tools, a powerful dimerization system to control receptor positioning, and physiological read-outs of synapse function.In this proposal I will combine innovative experimental and computational approaches, integrating single-molecule imaging with optical and electrophysiological measurements of neuronal function to: 1) elucidate the organizational principles that underlie the nano-compartmentalization of glutamate receptors at synapses, and 2) understand how the spatial distribution of receptor subtypes contributes to neuronal functioning. This project will reveal how nanoscale synapse organization contributes to neuronal circuit function, and will help understand how synaptic disruption contributes to neurological disease mechanisms. Dziedzina nauki natural sciencesbiological sciencesneurobiologycognitive neurosciencemedical and health sciencesclinical medicinepsychiatry Program(-y) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Temat(-y) ERC-2016-STG - ERC Starting Grant Zaproszenie do składania wniosków ERC-2016-STG Zobacz inne projekty w ramach tego zaproszenia System finansowania ERC-STG - Starting Grant Instytucja przyjmująca UNIVERSITEIT UTRECHT Wkład UE netto € 1 500 000,00 Adres HEIDELBERGLAAN 8 3584 CS Utrecht Niderlandy Zobacz na mapie Region West-Nederland Utrecht Utrecht Rodzaj działalności Higher or Secondary Education Establishments Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Uczestnictwo w unijnych programach w zakresie badań i innowacji Opens in new window sieć współpracy HORIZON Opens in new window Koszt całkowity € 1 500 000,00 Beneficjenci (1) Sortuj alfabetycznie Sortuj według wkładu UE netto Rozwiń wszystko Zwiń wszystko UNIVERSITEIT UTRECHT Niderlandy Wkład UE netto € 1 500 000,00 Adres HEIDELBERGLAAN 8 3584 CS Utrecht Zobacz na mapie Region West-Nederland Utrecht Utrecht Rodzaj działalności Higher or Secondary Education Establishments Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Uczestnictwo w unijnych programach w zakresie badań i innowacji Opens in new window sieć współpracy HORIZON Opens in new window Koszt całkowity € 1 500 000,00