Cel Symbiotic nitrogen fixation by rhizobia takes place in specialized organs of legume host plants, the root nodules. While rather deep molecular insights into plant-rhizobia recognition, early nodule organogenesis, regulation of nodulation and nitrogen fixation are available, much less is known about how nodule integrity is maintained and the origin of the underlying morphogenetic program. Recently, NOOT BOP COCH LIKE (NBCL) genes of Medicago truncatula and Pisum sativum were found to ensure nodule integrity by repressing ectopic root formation (applicant's former project). Interestingly, soybean nodules elicited by a Bradyrhizobium diazoefficiens mutant lacking the general stress response sigma factor σEcfG also formed ectopic roots (host's project), pointing to a bacteria-plant signalling system that is crucial for nodule persistence and integrity. Here, we propose to decipher the molecular determinants that maintain determinate nodule identity using the B. diazoefficiens – soybean model. We will combine plant molecular genetics, cell biology with transcriptomics and metabolomics to unravel cells, genes and metabolic networks that contribute to the checkpoint system ensuring nodule integrity. In work package 1 (WP1), the role of three soybean NBCL orthologs will be unravelled. In WP2, the nodule-to-root conversion characteristic for B. diazoefficiens ΔecfG mutants will be studied at the cellular level using root tip-specific reporter fusions. In WP3, a combination of metabolomics and transcriptomics will be applied to identify metabolites and gene networks involved in the nodule-to-root conversion elicited by ΔecfG and nbcl mutants. Altogether, results of this project will provide insight into determinants and mechanisms used by the economically important group of soybean plants to maintain the integrity of specialized, root-derived organs, an aspect that is relevant also in the light of ongoing attempts to engineer non-legumes into rhizobial hosts. Dziedzina nauki natural sciencesbiological sciencesmolecular biologymolecular geneticsnatural sciencesbiological sciencescell biologynatural sciencesbiological sciencesbiological behavioural sciencesethologybiological interactionsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymesagricultural sciencesagriculture, forestry, and fisheriesagriculturegrains and oilseedslegumes Słowa kluczowe Glycine max (soybean) Bradyrhizobium diazoefficiens symbiosis NOOT EcfG sigma factor homeosis nodule organogenesis and development Program(-y) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Temat(-y) MSCA-IF-2016 - Individual Fellowships Zaproszenie do składania wniosków H2020-MSCA-IF-2016 Zobacz inne projekty w ramach tego zaproszenia System finansowania MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Koordynator EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH Wkład UE netto € 187 419,60 Adres Raemistrasse 101 8092 Zuerich Szwajcaria Zobacz na mapie Region Schweiz/Suisse/Svizzera Zürich Zürich Rodzaj działalności Higher or Secondary Education Establishments Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Uczestnictwo w unijnych programach w zakresie badań i innowacji Opens in new window sieć współpracy HORIZON Opens in new window Koszt całkowity € 187 419,60