Objetivo Pluripotent Stem cells (PSCs) can give rise to all differentiated cells of the body and the germ line, which makes them conceptually fascinating and a valuable tool for regenerative medicine. Mouse PSCs are devoid of any developmental restriction partly thanks to their “open” chromatin, characterised by remarkably low levels of repressive epigenetic modifications. Metabolism is a key feature that can be adjusted to meet the cell’s needs, and that has the potential to feedback on transcription and epigenetics. How metabolism is regulated in PSCs and whether this is important for their biology remains largely unknown.We recently found a new molecular mechanism by which energy production is coupled to pluripotency. Here we propose to deepen our understanding of how metabolism, epigenetics and transcription are reciprocally regulated for the self-renewal and differentiation of PSCs. To gain insights into how metabolism is dynamically regulated in concert with the transcriptome and epigenome, we will also use somatic cell reprogramming into PSCs, a process in which both the metabolic and epigenetic profiles must be reset to match those of PSCs. Moreover, taking advantage of the recent generation of novel human PSCs sharing most of the transcriptional and epigenetic features found in naïve mouse PSCs, we will explore how metabolic regulatory mechanisms key for the generation and maintenance of pluripotency are conserved throughout evolution. Altogether, large-scale transcriptional, epigenetic and metabolic profiling of PSCs, combined with cutting edge technologies for their generation, expansion and genetic manipulation, will give us the unprecedented opportunity to build a comprehensive computational model of the metabolic network in PSCs, and to study how gene transcription and metabolism regulate each other. Ámbito científico medical and health sciencesmedical biotechnologygenetic engineeringmedical and health sciencesmedical biotechnologycells technologiesstem cellsnatural sciencesbiological sciencesgeneticsepigenetics Programa(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Tema(s) ERC-2016-STG - ERC Starting Grant Convocatoria de propuestas ERC-2016-STG Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-STG - Starting Grant Institución de acogida UNIVERSITA DEGLI STUDI DI PADOVA Aportación neta de la UEn € 1 498 232,00 Dirección VIA 8 FEBBRAIO 2 35122 Padova Italia Ver en el mapa Región Nord-Est Veneto Padova Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 1 498 232,00 Beneficiarios (1) Ordenar alfabéticamente Ordenar por aportación neta de la UE Ampliar todo Contraer todo UNIVERSITA DEGLI STUDI DI PADOVA Italia Aportación neta de la UEn € 1 498 232,00 Dirección VIA 8 FEBBRAIO 2 35122 Padova Ver en el mapa Región Nord-Est Veneto Padova Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 1 498 232,00