Objectif Nanopores have emerged in the past few years as a promising analytical technique. The basic concept of nanopore sensing is to apply a potential across individual nanoscale pores and observe the disruption of the ionic flow caused by single molecules entering the pore. Ionic currents through protein pores have been successful at recognizing tiny differences in molecules in solution. Most notably, arrays of thousands of nanopores integrated in low-cost and portable devices are now capable of sequencing DNA at the single-molecule level. The main challenge of nanopore sensing is the inability of controlling the protein pore diameter and geometry, which determines the signal and enables selectivity based on physical size.The aim of this proposal is to design a new generation of protein nanopores that will take on the next grand challenge in nanopore sensing, that is the sequence identification of single proteins. In order to sequence proteins, the designed nanopores must: unfold a target protein, control the speed of its transit across the nanopore and recognize individual amino acids. Our approach is to design a transmembrane molecular machine that will unfold target proteins and feed the linearize polypeptide through the nanopore where single amino acids will be recognized by modulations of the nanopore current.The specific objectives are: i) Develop chemical and biotechnological methods to design synthetic protein-based pores ii) To precisely attach the unfolding machine to a nanoporeiii) To genetically engineer the nanopore for optimal amino acid recognitionOur nanopore devices will be used to develop the first technology to sequence single proteins. Compared to the state of the art ‘shotgun proteomics’, the nanopore approach will allow long polypeptide reads, recognition of low-abundance proteins, including biomarkers linked to diseases, and real-time monitoring with minimal cost, time and sample preparation. Champ scientifique natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesbiological sciencesgeneticsDNAnatural sciencesmathematicspure mathematicsgeometrynatural scienceschemical sciencesorganic chemistryamines Mots‑clés DeE-Nano Programme(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Thème(s) ERC-2016-COG - ERC Consolidator Grant Appel à propositions ERC-2016-COG Voir d’autres projets de cet appel Régime de financement ERC-COG - Consolidator Grant Institution d’accueil RIJKSUNIVERSITEIT GRONINGEN Contribution nette de l'UE € 1 999 970,00 Adresse Broerstraat 5 9712CP Groningen Pays-Bas Voir sur la carte Région Noord-Nederland Groningen Overig Groningen Type d’activité Higher or Secondary Education Establishments Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 1 999 970,00 Bénéficiaires (1) Trier par ordre alphabétique Trier par contribution nette de l'UE Tout développer Tout réduire RIJKSUNIVERSITEIT GRONINGEN Pays-Bas Contribution nette de l'UE € 1 999 970,00 Adresse Broerstraat 5 9712CP Groningen Voir sur la carte Région Noord-Nederland Groningen Overig Groningen Type d’activité Higher or Secondary Education Establishments Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 1 999 970,00
