Obiettivo In eukaryotic cells mRNAs can be localized to specific places within a cell to achieve protein synthesis with spatiotemporal control. Recently, I and others have shown that prokaryotes can also target mRNAs to specific parts of the cell. However, these studies have been restricted to a limited number of mRNAs and were performed with Gram-negative bacteria, and due to the serendipitous nature of these discoveries and conflicting results, it is not clear how widespread mRNA localization is in a bacterial cell and what conserved mechanisms are playing a role. To investigate this, I will first employ a novel CLIP-seq-inspired deep-sequencing-based approach to gain an unbiased view of mRNAs enriched at the cell periphery and cell poles of the Gram-positive model bacterium Bacillus subtilis. This dataset will reveal the scope of mRNA targeting by the Signal Recognition Particle (SRP) and will reveal mRNAs that use another mechanism for cellular targeting. In addition, classic CLIP-seq experiments will be used to identify mRNAs that use the cytoskeleton and other morphological proteins for targeting. As a complementary approach, I will assess the localization of mRNAs coding for different classes of localized proteins (polar, cell division, cytoskeletal, membrane, secreted, motility, nucleoid and chaperone proteins) using the established fluorescence in situ hybridization (FISH) technique. Information obtained from these experiments will subsequently be used to investigate the role of protein translation, RNA sequences, genome location and cell morphology in mRNA localization, to gain functional insight into the mechanisms responsible for this basic morphogenetic process. Campo scientifico natural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesgeneticsRNAnatural sciencesbiological sciencesgeneticsgenomes Parole chiave RNA localization Cytoskeleton RNA binding proteins Programma(i) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Argomento(i) MSCA-IF-2016 - Individual Fellowships Invito a presentare proposte H2020-MSCA-IF-2016 Vedi altri progetti per questo bando Meccanismo di finanziamento MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinatore UNIVERSITEIT VAN AMSTERDAM Contribution nette de l'UE € 165 598,80 Indirizzo SPUI 21 1012WX Amsterdam Paesi Bassi Mostra sulla mappa Regione West-Nederland Noord-Holland Groot-Amsterdam Tipo di attività Higher or Secondary Education Establishments Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Costo totale € 165 598,80
