Cel Recent breakthroughs in the field of genome editing provide a genuine opportunity to establish innovativeapproaches to repair DNA mutations to replace, engineer or regenerate malfunctioning cells in vitro or invivo. However, most of the recently developed technologies introduce double-strand DNA breaks at a targetlocus as the first step to gene correction. These breaks are subsequently repaired by one of the cell intrinsicDNA repair pathways, typically inducing an abundance of insertions and deletions (indels). Ideally, for manyapplications genome editing should, however, be efficient and specific, without the introduction of indels.Site-specific recombinases (SSRs) allow precise genome editing without triggering endogenous DNA repairpathways and possess the unique ability to fulfill both cleavage and immediate resealing of the processedDNA in vivo. However, customizing the DNA binding specificity of SSRs is not straightforward. With thisproject, we propose to solve this shortcoming. We have already demonstrated that by applying substrate-linkeddirected evolution, SSRs can be generated that specifically recognize therapeutic targets. Theobjective of this project is the development of a universal genome editing platform that allows flexible,efficient and safe gene corrections in cells of any origin without triggering cell intrinsic DNA repair.GenSurge aims to: i) sequence an unprecedented, comprehensive compendium of evolved SSRs tounderstand the directed molecular evolution process at nucleotide resolution; ii) integrate the knowledgeobtained in i) to develop a unique SSR-based approach to correct genomic inversions; iii) develop auniversal SSR-based strategy that allows flawless, precise and safe genome editing to correct any gene defectin human, animal or plant cells. The successful implementation of this project will deliver a comprehensive,safe and efficient platform from which genome surgery-based cure strategies can be initiated. Dziedzina nauki natural sciencesbiological sciencesgeneticsgenomes Słowa kluczowe genetic engineering genome editing side-specific recombination Program(-y) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Temat(-y) ERC-2016-ADG - ERC Advanced Grant Zaproszenie do składania wniosków ERC-2016-ADG Zobacz inne projekty w ramach tego zaproszenia System finansowania ERC-ADG - Advanced Grant Instytucja przyjmująca TECHNISCHE UNIVERSITAET DRESDEN Wkład UE netto € 2 380 425,00 Adres HELMHOLTZSTRASSE 10 01069 Dresden Niemcy Zobacz na mapie Region Sachsen Dresden Dresden, Kreisfreie Stadt Rodzaj działalności Higher or Secondary Education Establishments Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Uczestnictwo w unijnych programach w zakresie badań i innowacji Opens in new window sieć współpracy HORIZON Opens in new window Koszt całkowity € 2 380 425,00 Beneficjenci (1) Sortuj alfabetycznie Sortuj według wkładu UE netto Rozwiń wszystko Zwiń wszystko TECHNISCHE UNIVERSITAET DRESDEN Niemcy Wkład UE netto € 2 380 425,00 Adres HELMHOLTZSTRASSE 10 01069 Dresden Zobacz na mapie Region Sachsen Dresden Dresden, Kreisfreie Stadt Rodzaj działalności Higher or Secondary Education Establishments Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Uczestnictwo w unijnych programach w zakresie badań i innowacji Opens in new window sieć współpracy HORIZON Opens in new window Koszt całkowity € 2 380 425,00