Project description DEENESFRITPL Innovative Raman endoscopy for real-time diagnosis of bladder cancer Raman endoscopy is a label-free optical technique that provides a molecular fingerprint of tissue for cancer diagnosis in vivo. Presently, state-of-the-art Raman endoscopy does not provide compositional analysis or insights into the molecular biology of tissue. The EU-funded ENDOMICS project aims to create a new paradigm of this technology using mass spectrometry that enables proteomic and lipidomic analysis for the diagnosis of bladder cancer. The project will also develop a fibre-optic Raman endoscope that can measure depth-dependent molecular profiles for simultaneous detection, grading and staging of bladder cancer. The final clinical trial will allow the identification of proteomic and lipidomic biomarkers to develop novel algorithms for real-time diagnosis of bladder cancer. Show the project objective Hide the project objective Objective The goal of ENDOMICS is to drive forward a new paradigm of Raman endoscopic technology that enables proteomic and lipidomic analysis for diagnosis of bladder cancers in vivo. Raman endoscopy is a label-free optical technique that can provide a point-wise vibrational molecular fingerprint of tissue “optical biopsy” for cancer diagnosis in vivo. State-of-the-art Raman endoscopy, however, does not offer specific compositional analysis or insights into molecular biology of tissue. This is because the vibrational Raman bands are overlapping and cannot be deciphered into the myriad of biomolecules in complex tissue. We will introduce a ground-breaking new methodology to enable Raman proteomic and lipidomic analysis in vivo. To this end, heterospectral co-registered Raman and mass spectrometry imaging will be used to develop a multivariate regression model “Rosetta Stone” for translating vibrational structural information (Raman spectroscopy) into compositional information. To meet the unmet clinical needs in urology we will tailor the first fibre-optic Raman endoscopic technology that can measure depth-dependent molecular profiles to simultaneously enable detection, grading and staging of bladder cancers. We will finally conduct a clinical trial by applying the technique to measure a comprehensive molecular database of bladder pathologies in vivo. The latter will allow for the identification of proteomic and lipidomic biomarkers to develop novel algorithms for real-time diagnosis of bladder cancers. The synergy between scientific and technological advances in ENDOMICS will break ground for shedding new light on the molecular biology of bladder cancer in vivo including new insights into clinical diversity and identification of biomarkers for diagnostics, prognostics and novel therapeutic targets. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsmedical and health sciencesclinical medicineoncologybladder cancermedical and health sciencesclinical medicineurologymedical and health sciencesbasic medicinepathologynatural sciencesbiological sciencesmolecular biology Keywords Raman proteomics Raman lipidomics biomedical spectroscopy bladder cancer cystoscopy in vivo diagnosis Programme(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Topic(s) ERC-2018-STG - ERC Starting Grant Call for proposal ERC-2018-STG See other projects for this call Funding Scheme ERC-STG - Starting Grant Host institution KING'S COLLEGE LONDON Net EU contribution € 1 490 950,00 Address STRAND WC2R 2LS London United Kingdom See on map Region London Inner London — West Westminster Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 1 490 950,00 Beneficiaries (1) Sort alphabetically Sort by Net EU contribution Expand all Collapse all KING'S COLLEGE LONDON United Kingdom Net EU contribution € 1 490 950,00 Address STRAND WC2R 2LS London See on map Region London Inner London — West Westminster Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 1 490 950,00
