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Contenu archivé le 2022-12-23

Studies on radiation induced chromosomal aberrations

Objectif



In this extended project, covering East European countries, some aspects of basic mechanisms of radiation induced chromosome aberrations in human and other mammalian cells will be investigated. The proposed research projects aim at giving information on areas not covered by the original project and at the same time attempting to transfer modern technology to the East European Laboratories. From the studies carried out in Leiden, it is clear that inhibitors of DNA repair increase the frequency of dicentrics but not the translocations in human lymphocytes induced by X-rays, indicating that different mechanisms may be involved in the formation of stable and unstable aberrations. Under this project, studies will be made to check whether radio-protectors, which reduce the frequencies of X-ray induced dicentrics have any influence on the yield of translocations using chromosome painting technique (sofia). It has also been found that in addition to reciprocal translocations, X-rays induce interstial and terminal translocations in human and mouse lymphocytes (Leiden). It is important to see whether these different types of translocations are induced in the male germ cells and how they behave with regard to pairing in diakinesis stage of male germ cells (sofia). Mouse chromosome specific DNA libraries have already been generated in Leiden. The radiation quality factor in relation to induction of translocations and dicentrics has not been extensively investigated. Studies in Leiden have indicated that with 1 MeV neutrons, the dicentric yields and translocation yields are not very different, especially at low doses, which has implications with regard to RBE for induction of dicentrics us translocations. To extend this observation studies will be conducted with 0.2 to 5 MeV neutrons generated by a Triga Mark II reactor (Ljublijana, Slovenia). In addition, the effectiveness of heavy ions (protons, deuterons,3HE,4HE ions) with LET ranging from 0.3t0 50 kev/um in induction of dicentrics and translocations will be evaluated and compared to the yields obtained with gamma rays (Brno). Fluorescentin situ hybridization (FISH)technique using chromosome specific DNA libraries will be introduced to the participating laboratories by the Leiden group. The results obtained by these studies are expected to contribute to the understanding of basic mechanisms involved in the

Appel à propositions

Data not available

Régime de financement

CSC - Cost-sharing contracts

Coordinateur

Rijksuniversiteit Leiden
Contribution de l’UE
Aucune donnée
Adresse
72,Wassenaarseweg
2300 RA Leiden
Pays-Bas

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Coût total
Aucune donnée

Participants (1)