Objectif Adenosine, a metabolite whose production is increased during metabolic inhibition, is known to exert inhibitory effects on heart. Recently, however, it has been shown that low concentrations of adenosine (Ado) increase automaticity in the isolated right ventricle of the rat, whereas inhibition is seen at high concentrations. Both effects are antagonized by theophylline, suggesting interaction with an extracellularly located Ado receptor. The aim of the present project is to analyse at different levels the stimulatory effect of Ado: 1) characterization of the Ado receptor by radioligand studies; 2) pharmacological study of the interaction with adrenergic receptors; 3) analysis of the underlying electrophysiological changes in terms of ionic currents at the single cell level (patch electrode) and of the eventual concomitant changes in intracellular Ca concentration, using the indo-fluorescence method. Programme(s) FP2-SCIENCE - Programme plan (EEC) to stimulate the international cooperation and interchange needed by European research scientists (SCIENCE), 1988-1992 Thème(s) Data not available Appel à propositions Data not available Régime de financement CSC - Cost-sharing contracts Coordinateur Katholieke Universiteit Leuven Contribution de l’UE Aucune donnée Adresse 59,Naamsestraat 3000 Leuven Belgique Voir sur la carte Coût total Aucune donnée Participants (2) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire Fundação Calouste Gulbenkian Portugal Contribution de l’UE Aucune donnée Adresse 14,Rua Da Quinta Grande 2781 Oeiras Voir sur la carte Coût total Aucune donnée Universidad de Murcia Espagne Contribution de l’UE Aucune donnée Adresse Espinardo 30100 Murcia Voir sur la carte Coût total Aucune donnée
