Obiettivo The objective is to determine, before the end of 1997, the entire genome sequence of Bacillus subtilis which is both of industrial importance and a model organism for Gram-positive bacteria. An international agreement has been reached allowing the allocation of regions of the chromosome among the groups of the European and Japanese Bacillus subtilis genome sequencing networks. This international project currently includes 17 European and 7 Japanese laboratories, together with 2 biotechnology companies (Genencor International and Novo Nordisk) and 1 Korean laboratory.The European sequencing groups have obtained support from the European Commission (this contract and contract BIO2 CT-930272). The initial objective of this project (contract BIO2 CT-942011) was to determine the DNA sequence of about 10 % of the Bacillus subtilis genome (0.45 Mbp). To match this deadline, three high throughput sequencing groups have recently been integrated in this contract as subcontractors (see below).A cloning strategy for the construction of gene banks representative of the entire B.acillus subtilis chromosome is not available. This is, at least in part, due to the generally high level of expression of Bacillus subtilis genes observed in Escherichia coli, leading to toxic effects in this commonly used cloning host. However, participating groups have employed a variety of strategies to overcome this limitation. They include: (1) cloning into a range of plasmid, lambda and YAC vectors; (2) the use of an Escherichia coli strain that lowers the copy number of ColE1-based plasmids and which correspondingly reduces the potential toxic effects of cloned genes; (3) the use of integrative plasmids and marker rescue in genome walking experiments; (4) in vitro amplification using standard, long-range (LR PCR) and inverse PCR techniques.MAJOR SCIENTIFIC BREAKTHROUGHS AND INDUSTRIAL APPLICATIONS:During this contract 0.74 Mbp of Bacillus subtilis DNA has been sequenced. The European sequencing network has presently deposited a total of 1.9 Mbp of new B. subtilis DNA sequence at the database maintained by the Institut Pasteur, confirming the leader position of the EU in the systematic sequencing of this genome. In the current EU framework, a relational database containing 3.6 Mbp of non redundant B. subtilis DNA sequences, representing 86 % of the total genome, has been constructed at the Institut Pasteur.An industrial platform has been set up to facilitate contacts between project participants and European biotechnology companies. The following companies have agreed to this mode of cooperation: DuPont de Nemours (France), Genencor (Finland, USA), F. Hoffmann-La Roche AG (Switzerland), Novo Nordisk (Denmark), Puratos (Belgium), Roussel Uclaf (France), SmithKline Beecham Pharmaceuticals (United Kingdom). Moreover, a confidential document containing a list of similarities of B. subtilis proteins with known gene products is forwarded by the coordinator, at regular intervals, to the companies mentioned above. Campo scientifico medical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsnatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesgeneticschromosomesnatural sciencesbiological sciencesgeneticsgenomesnatural sciencescomputer and information sciencesdatabasesrelational databases Programma(i) FP3-BIOTECH 1 - Specific research and technological development programme (EEC) in the field of biotechnology, 1990-1994 Argomento(i) Data not available Invito a presentare proposte Data not available Meccanismo di finanziamento CSC - Cost-sharing contracts Coordinatore INSTITUT PASTEUR Contributo UE Nessun dato Indirizzo Rue du Docteur Roux 25 75724 PARIS Francia Mostra sulla mappa Costo totale Nessun dato Partecipanti (7) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto BBSRC Institute of Food Research Regno Unito Contributo UE Nessun dato Indirizzo Reading Laboratory Earley Gate Whiteknights Road RG6 2EF Reading Mostra sulla mappa Costo totale Nessun dato CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE Francia Contributo UE Nessun dato Indirizzo Chemin Joseph Aiguier 31 13402 Marseille Mostra sulla mappa Costo totale Nessun dato Humboldt-Universität zu Berlin Germania Contributo UE Nessun dato Indirizzo 42,Invalidenstrasse 10115 Berlin Mostra sulla mappa Costo totale Nessun dato Johann-Wolfgang-Goethe Universitat Frankfurt Germania Contributo UE Nessun dato Indirizzo Marie Curie Straße 9 60439 Frankfurt am Main Mostra sulla mappa Costo totale Nessun dato UNIVERSITE DE LIEGE Belgio Contributo UE Nessun dato Indirizzo 17,Allee de la Chimie 3 - Bètiment B6 4000 LIEGE Mostra sulla mappa Costo totale Nessun dato UNIVERSITE DE PARIS-SUD XI Francia Contributo UE Nessun dato Indirizzo Rue Georges Clemenceau 15, BFtiment 400 91405 GOMETZ LA VILLE Mostra sulla mappa Costo totale Nessun dato VRIJE UNIVERSITEIT AMSTERDAM - VERENIGING VOOR CHRISTELIJK WETENSCHAPPELIJK ONDERWIJS Paesi Bassi Contributo UE Nessun dato Indirizzo 1087,De Boelelaan 1087 1081 HV AMSTERDAM Mostra sulla mappa Costo totale Nessun dato