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Contenido archivado el 2022-12-23

MOLECULAR ANALYSIS OF BIO-SYNTHETIC PATHWAYS INVOLVED IN PRODUCT ACCUMULATION IN LEGUME SEEDS MANIPULATIONS TO IMPROVE SEED COMPOSITION

Objetivo


Plastids were isolated from the cells of developing pea embryos for studies of the substrates for starch synthesis and for fatty acid and amino acid synthesis.

It was established that glucose-6-phosphate enters the plastids of developing pea embryos as the substrate for starch synthesis and it does so via a specific exchange translocator which also catalyzes the movement of triose phosphate and inorganic phosphate. Inside the plastid, glucose-6-phosphate may also be metabolised via the oxidative pentose phosphate pathway (OPPP). The extent of this metabolism is dependent upon the demand for reductant, a major component of which may be the synthesis of glutamate.

Radioactivity studies showed that glucose-6-phosphate is probably not the substrate for amino acid synthesis in plastids suggesting that some other intermediate may enter the plastid to fill this role. Also, the synthesis of fatty acids does not represent an important fate for glucose-6-phosphate inside plastids of pea embryos, whose rate of lipid synthesis is very low compared with the rate of starch synthesis.

Tema(s)

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Régimen de financiación

CSC - Cost-sharing contracts

Coordinador

JOHN INNES CENTRE
Aportación de la UE
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Dirección
Norwich Research Park, Colney
NORWICH
Reino Unido

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