Cel The objectives of this project are: the development of methods for the early screening of resistant varieties in the model system tomato - Fusarium oxysporum; to study the regenerating capacity of plant cell cultures as a prerequisite for in vitro selection and to investigate the molecular aspects of Beta glucanase production in host-parasite interactions in both the plant and the fungus. On the basis of previous experience that points to a critical role for polysaccharides in active defence, a method has been developed and used to select tomato cell cultures that differ from each other in their callose content. The clones obtained were further tested for other biochemical parameters known to be included in active defence such as peroxidase synthesis in dual culture with the pathogen; and inhibition of the pathogens in dual culture. Three commercial varieties of tomato (UC 105, Marmande and Tondino) were tested under different hormonal balances in order to establish the regenerating capacity and the best hormonal conditions for the production of callus as well as the best medium for regeneration of cell cultures. Tondino has shown surprisingly high regenerative capacity and the hormonal requrirements have been established for its regeneration from cell cultures. This method and this particular genotype will be used for regenerating plants from cells selected for their high polysaccharide content and will be further tested for in vivo resistance to Fusarium. A full comprehension of host pathogen interaction implies an understanding of the patterns of gene activations of both partners from recognition onwards. Earlier results revealed that plant tissue may respond to pathogens through amplification of specific sequences. Work is at present being undertaken on the amplification and transcription of tomato. To start a line of research on gene activation and amplification in the fungus, a genomic library of Fusarium oxysporum fsp lycopersici was constructed in the phagic vector EMBL3. The library is now being used to isolate sequences belonging to the structural genes known to be involved in virulence processes. Program(-y) IC-ISC C - Activity (Euratom, EEC) on cooperation in science and technology, 1984- Temat(-y) Data not available Zaproszenie do składania wniosków Data not available System finansowania CSC - Cost-sharing contracts Koordynator UNIVERSITY OF FLORENCE Wkład UE Brak danych Adres Via Romana 17/19 50125 FIRENZE Włochy Zobacz na mapie Koszt całkowity Brak danych
