Objetivo Cryo-preservation of animal germ plasm is an attractive complement to the indispensable maintenance of genetic diversity through living animals.Techniques are now available to freeze store both sperm and embryos of several domestic species. However their efficiencies and cost of utilisation under routine conditions are at the moment not defined. Research is needed to effectively employ these techniques in a multidisciplinary program aimed at the preservation of the numerous subpopulations of animals that exist within a given species.Cryopreservation of animal germplasm is an attractive complement to the indispensable maintenance of genetic diversity through living animals. However, research is needed to effectively apply these techniques in a multidisciplinary programme aimed at the preservation of the numerous subpopulations of animals that exist within a given species. A pilot project has been set up using the rabbit as a research model. The research involves standardization of the procedure used for embryos and sperm storage, the calculation of the size of the sample to be frozen (number of embryos, and/or semen doses) to guarantee the reestablishment of a line with relatively few representatives, and the definition of the genetic requirement needed for corrects sampling of the selected strains to be stored. A procedure has been designed that allows more than 50% of the frozen stored embryos to develop into living offspring following their transfer into foster recipients. This procedure is however time consuming and efforts towards simplification have been engaged using vitrification (ultrarapid freezing). A line (A-1077) selected during numerous generations for litter size and maintained as a close breeding group is proposed as reference for measuring the technical efficiency of cryobanking, this in order to offer a first guarantee to the potential future users of the bank.The proposal is aimed towards the effective utilization of gamete and embryo cryobanking for the preservation and exploration of genetic diversity in a domestic species. Rabbit has been chosen as an experimental model because it allows access to different and numerous subpopulation categories with less constraints than with other major species of similar economic value. Three research groups already engaged in ex-situ conservation of rabbit germplasm are willing to participate in this concerted action which will examine on an experimental basis both technical, genetic, economical and legal aspects of germplasm cryobanking. Particular attention will be given to the standardisation of storage conditions, to the sampling of genetic material for each rabbit population concerned and to the availability of genetic information through a data base network. Ámbito científico agricultural sciencesanimal and dairy sciencedomestic animalsmedical and health sciencesclinical medicineembryology Programa(s) FP3-BIOTECH 1 - Specific research and technological development programme (EEC) in the field of biotechnology, 1990-1994 Tema(s) 3.2 - Conservation of genetic resources Convocatoria de propuestas Data not available Régimen de financiación CON - Coordination of research actions Coordinador Institut National de la Recherche Agronomique (INRA) Aportación de la UE Sin datos Dirección Domaine de Vilvert 78352 Jouy-en-Josas Francia Ver en el mapa Coste total Sin datos Participantes (2) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo Ludwig-Maximilians-Universität München Alemania Aportación de la UE Sin datos Dirección Veterinärstraße 13 80539 München Ver en el mapa Coste total Sin datos UNIDAD DE FISIOLOGIA España Aportación de la UE Sin datos Dirección CAMINO DE VERA 14 46071 VALENCIA 22012 Ver en el mapa Coste total Sin datos
