Objetivo Septic shock is a severe and frequent clinical complication which causes about 50000 deaths per year in western europe. The underlying pathogenic mechanism is still unclear. Enhanced levels of nitrogen monoxide (NO) in the cardiovascular system are suspected to cause the hyporeactivity to vasoconstricting agents that underlies the unrelenting hypotension associated with septic shock. Prof. Vanin and coworkers now propose to study the kinetics and organ distribution of NO formation in an animal model of endotoxemia to evaluate the integrated response of an intact organism to septic shock. Furthermore, they intend to assess the role of proand antioxidants in this response, to gain insight into potentially beneficial pharmacologic approaches towards an treatment of septic shock. They will investigate a recent idea that induction of NO synthase may selectively be prevented by inhibitors of the transcription factor NFkappaB, and that non-heme iron is one important intracellular signal that is blocked by this approach. To analyze a potentially organo-toxic effect of enhanced NO formation the concentration of iron-sulfur centers and dinitrosyl iron complexes in the tissues will be measured simultaneously. Prof. Vanin's group has a long-standing experience in measurement of NO levels in tissues obtained from living animals, as well as in assessment of non-heme iron content and detection of paramagnetic nitrosylation products. Prof. Vanin's laboratory is equipped with all technical facilities required for this study. The intended cooperation will significantly strengthen the technical capabilites and extend the mechanism-based research in the field of NO synthase induction existing in other laboratories of this Biomed 1 project. Programa(s) IC-PECO/COPERNICUS - Scientific and technological cooperation between the European Community and European non-member countries, 1992- Tema(s) 0201 - CEEC participation in BIOMEDICAL AND HEALTH RESEARCH programme Convocatoria de propuestas Data not available Régimen de financiación CSC - Cost-sharing contracts Coordinador Albert-Ludwigs-Universität Freiburg Aportación de la UE Sin datos Dirección Herman-Herder-Straße 7 79104 Freiburg Alemania Ver en el mapa Coste total Sin datos Participantes (2) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo Institute of Chemical Physics - Russian Academy of Sciences Rusia Aportación de la UE Sin datos Dirección 4,Kosygina 117334 Moskva Ver en el mapa Coste total Sin datos Jagellonian University Polonia Aportación de la UE Sin datos Dirección 15 A,Kopernika 31 501 Kraków Ver en el mapa Coste total Sin datos
