Cel Present techniques for the identification of fish species, especially tunas are unable to identify fish that has been canned. The nucleic acid sequences of the fish offer a unique and stable foundation on which to base a method for the differentiation of closely related tuna species. The objective is to develop DNA/RNA-based diagnostic techniques to identify individual tuna and bonito species in commercially canned products : 1. Collection, authentication and canning of individual tuna and bonito species2. Extraction of DNA from canned samples and sequencing a variable region of the mitochondrial cytochrome b gene in accordance with published methods. 3. Evaluation of other nucleic acid technologies 3.1. Probes for fragments of the mitochondrial cytochrome b gene 3.2. Restriction enzyme fragments of large molecular weight DNA 3.3. Sequencing of ribosomal RNA 3.4. Random primer amplified polymorphic DNA profiles 3.5. Restriction enzyme fragments of mitochondrial DNA 4. Selection and optimisation of the most appropriate technique 5. Validation of the technique and comparison with the best alternative technology. Dziedzina nauki natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological scienceszoologyichthyologynatural sciencesbiological sciencesgeneticsRNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Program(-y) FP3-AIR - Specific research and technological development and demonstration programme (EEC) in the field of agriculture and agro-industry, including fisheries, 1990-1994 Temat(-y) 416 - Prenormative food research Zaproszenie do składania wniosków Data not available System finansowania CSC - Cost-sharing contracts Koordynator Ministry of Agriculture, Fisheries and Food (MAFF) Wkład UE Brak danych Adres 135 Abbey Road AB9 8DG Aberdeen Zjednoczone Królestwo Zobacz na mapie Koszt całkowity Brak danych Uczestnicy (3) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko Bundesforschungsanstalt für Fischerei Niemcy Wkład UE Brak danych Adres Palmaille 9 22767 Hamburg Zobacz na mapie Koszt całkowity Brak danych CSIC Hiszpania Wkład UE Brak danych Adres SERRANO 117 28006 VIGO Zobacz na mapie Koszt całkowity Brak danych UNIVERSIDAD DE SANTIAGO DE COMPOSTELA Hiszpania Wkład UE Brak danych Adres PAZO DE SAN XEROME 15705 SANTIAGO DE COMPOSTELA Zobacz na mapie Koszt całkowity Brak danych
